Ọrụ ugbo bụ ihe kachasị mkpa n'ahịa ụwa, usoro gburugburu ebe obibi na-echekwa ọtụtụ ihe ịma aka. Iri fatịlaịza kemịkalụ n'ụwa niile na-eto ma na-arụ ọrụ dị mkpa na mkpụrụ osisi1. Agbanyeghị, osisi ndị a kụrụ n'ụzọ a enweghị oge zuru oke iji too ma tozuo nke ọma, ya mere ha anaghị enweta àgwà osisi dị mma2. Na mgbakwunye, ihe ndị na-egbu egbu nwere ike ịgbakọta n'ahụ mmadụ na ala3. Ya mere, ọ dị mkpa ịmepụta ngwọta ndị na-adịghị ize ndụ gburugburu ebe obibi na nke na-adịgide adịgide iji belata mkpa maka fatịlaịza kemịkalụ. Ihe ndị na-adịghị ndụ bara uru nwere ike ịbụ isi iyi dị mkpa nke ihe ndị sitere n'okike na-arụ ọrụ na bayoloji4.
Ọgbakọ ndị nwere mkpụrụ ndụ ihe nketa dị iche iche dabere na ụdị osisi ma ọ bụ ụdị mkpụrụ ndụ ihe nketa, oge uto osisi, na ọdịdị osisi. 13 Ọtụtụ ọmụmụ akọpụtala na Azospirillum, Bacillus, Azotobacter, Pseudomonas, na Enterobacter nwere ike ime.kwalite uto osisi. 14 Ọzọkwa, Bacillus na Azospirillum bụ mkpụrụ PGPB kachasị amụ n'ihe gbasara ime ka uto na mkpụrụ osisi ka mma. 15 Ọmụmụ ihe egosila na ịgbakọ Azospirillum brasiliensis na Bradyrhizobium n'ime akwụkwọ nri nwere ike ime ka mkpụrụ ọka, ọka wit, soybean, na agwa akụrụ dịkwuo mma. 16, 17 Ọmụmụ ihe egosila na ịgba Salicornia na Bacillus licheniformis na PGPB ndị ọzọ na-akwalite uto osisi na oriri nri. 18 Azospirillum brasiliensis Sp7 na Bacillus sphaericus UPMB10 na-eme ka uto mgbọrọgwụ nke unere dị ụtọ ka mma. N'otu aka ahụ, mkpụrụ fennel siri ike itolite n'ihi uto ahịhịa na-adịghị mma na obere mkpụrụ, karịsịa n'okpuru ọnọdụ nrụgide ọkọchị20. Ọgwụgwọ mkpụrụ na Pseudomonas fluorescens na Trichoderma harzianum na-eme ka uto mbụ nke mkpụrụ fennel dịkwuo mma n'okpuru ọnọdụ nrụgide ọkọchị21. Maka stevia, e meela nnyocha iji nyochaa mmetụta nke ero mycorrhizal na uto osisi na-akwalite rhizobacteria (PGPR) na ikike nke ihe dị ndụ itolite, ịchịkọta metabolites nke abụọ, na mkpụrụ ndụ ihe nketa dị na biosynthesis. Dịka Rahi et al.22 si kwuo, ịgba ọgwụ osisi nwere PGPR dị iche iche mere ka uto ha ka mma, photosynthetic index, na nchịkọta nke stevioside na stevioside A. N'aka nke ọzọ, ịgba ọgwụ stevia na rhizobia na-akwalite uto osisi na ero mycorrhizal arbuscular kpaliri ịdị elu osisi, stevioside, mineral, na ọdịnaya pigment.23 Oviedo-Pereira et al.24 kọrọ na endophytes na-akpasu iwe Enterobacter hormaechei H2A3 na H5A2 mụbara ọdịnaya SG, kpalite njupụta trichome na akwụkwọ, ma kwalite mkpokọta metabolites kpọmkwem na trichomes, mana ha akwaliteghị uto osisi;
GA3 bụ otu n'ime protein ndị dị mkpa ma na-arụ ọrụ nke ọma dịka gibberellin31. Ọgwụgwọ stevia site na GA3 nwere ike ime ka ogologo osisi na okooko dịkwuo elu32. N'aka nke ọzọ, ụfọdụ ọmụmụ egosila na GA3 bụ ihe na-akpali osisi imepụta metabolites nke abụọ dịka antioxidants na pigments, ọ bụkwa usoro nchekwa33.
Mmekọrịta phylogenetic nke ihe ndị dị ndụ na-ejikọta ya na ụdị ụdị nje ndị ọzọ. E nyere ọnụọgụ njikọta GenBank n'ime akara aka.
A na-egosi ọrụ Amylase, cellulase na protease dị ka eriri doro anya gburugburu colonies, ebe ihe ndị na-agbapụta ọcha gburugburu colonies na-egosi ọrụ lipase. Dịka egosiri na Tebụl 2, B. paramycoides SrAM4 nwere ike imepụta hydrolases niile, ebe B. paralicheniformis SrMA3 nwere ike imepụta enzymes niile ma e wezụga cellulase, B. licheniformis SrAM2 na-emepụta naanị cellulase.
Ọtụtụ mkpụrụ ndụ nje dị mkpa ejikọtala na mmụba nke njikọta metabolite nke abụọ na osisi ọgwụ na aromatic74. Antioxidants niile dị mkpa na nke na-abụghị enzymatic mụbara nke ukwuu na S. rebaudiana Shou-2 ma e jiri ya tụnyere njikwa ahụ. Chamam et al.75 kọkwara mmetụta dị mma nke PGPB na TPC na osikapa; Ọzọkwa, nsonaazụ anyị kwekọrọ na nsonaazụ nke TPC, TFC, na DPPH na S. rebaudiana, nke e kwuru na ọ bụ n'ihi njikọ Piriformospora indica na Azotobacter chroococcum76. TPC na TFC77 dị elu nke ukwuu na osisi basil ejiri microorganisms gwọọ ma e jiri ya tụnyere osisi ndị a na-agwọghị. Ọzọkwa, mmụba nke antioxidants nwere ike ime maka ihe abụọ: enzymes hydrolytic na-akpali usoro nchekwa osisi nke a kpaliri n'otu ụzọ ahụ dị ka microorganisms pathogenic ruo mgbe osisi ahụ gbanwere na nhazi nje bacteria78. Nke abụọ, PGPB nwere ike ịrụ ọrụ dị ka onye malitere mkpali nke ogige bioactive nke e guzobere site na ụzọ shikimate na osisi dị elu na microorganisms 79.
Nsonaazụ gosiri na e nwere mmekọrịta dị n'etiti ọnụọgụ akwụkwọ, ngosipụta mkpụrụ ndụ ihe nketa na mmepụta SG mgbe e tinyere ọtụtụ ụdị ọgwụ n'otu oge. N'aka nke ọzọ, ịgba ọgwụ ugboro abụọ dị mma karịa ịgba ọgwụ n'otu oge n'ihe gbasara uto na mmepụta osisi.
A chọpụtara enzymes hydrolytic mgbe a gbasịrị nje bacteria n'ime agar medium nke nwere ihe ngosi substrate ma tinye ha na okpomọkụ na 28 Celsius ruo ụbọchị 2-5. Mgbe e tinyere nje bacteria na starch agar medium, e jiri iodine 100 solution chọpụta ọrụ amylase. E jiri 0.2% mmiri Congo red reagent chọpụta ọrụ cellulase dịka usoro Kianngam et al. 101 si dị. A hụrụ ọrụ protease site na mpaghara doro anya gburugburu colonies e ji skim milk agar medium mee dịka Cui et al. 102 si kọwaa. N'aka nke ọzọ, a chọpụtara lipase 100 mgbe a gbasịrị ya na Tween agar medium.
Oge ozi: Jenụwarị-06-2025