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Exogenous gibberellic acid na benzylamine na-agbanwe uto na kemịkalụ nke Schefflera dwarfis: nyocha nkwụghachi azụ nke nzọụkwụ.

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Osisi nwa ehi na-achọ mma nwere ọdịdị mara mma na-eji kpọrọ ihe nke ukwuu.Otu ụzọ isi nweta nke a bụ ijindị na-ahụ maka uto osisidị ka ngwá ọrụ nlekọta ihe ọkụkụ.Emere ọmụmụ ihe ahụ na Schefflera dwarf (osisi ahịhịa ịchọ mma) ejiri ọgwụ na-agba agba.gibberellic acidna benzyladenine hormone na griin haus nke nwere usoro ogbugba mmiri n'ụgbụgbọ.A na-efesa homonụ ahụ na akwụkwọ dwarf schefflera na mkpokọta 0, 100 na 200 mg / l n'ime usoro atọ kwa ụbọchị iri na ise.Emere nnwale ahụ n'usoro ihe n'usoro n'usoro n'ụzọ zuru oke yana mmegharị anọ.Nchikota gibberellic acid na benzyladinine na ntinye nke 200 mg / l nwere mmetụta dị ukwuu na ọnụ ọgụgụ akwụkwọ, ebe akwukwo na elu osisi.Ọgwụgwọ a rụpụtakwara n'ọdịnaya kachasị elu nke pigmenti foto synthetic.Na mgbakwunye, ọnụ ọgụgụ kachasị elu nke carbohydrates soluble na mbelata shuga ka a na-ahụ benzyladenine na 100 na 200 mg/L na gibberellic acid + benzyladenine na 200 mg/L.Nnyocha nyocha nke nzọụkwụ na-egosi na olu mgbọrọgwụ bụ mgbanwe mbụ ịbanye na ihe nlereanya ahụ, na-akọwa 44% nke mgbanwe ahụ.Ngbanwe na-esote bụ nnukwu mgbọrọgwụ ọhụrụ, yana ụdị bivariate na-akọwa 63% nke mgbanwe dị na ọnụọgụ akwụkwọ.Mmetụta dị mma kachasị na ọnụọgụ akwụkwọ bụ site na ibu mgbọrọgwụ ọhụrụ (0.43), nke jikọtara ya na nọmba akwukwo (0.47).Nsonaazụ gosiri na gibberellic acid na benzyladenine na ntinye nke 200 mg / l mere ka ọganihu morphological dịkwuo mma, chlorophyll na carotenoid njikọ nke Liriodendron tulipifera, ma belata ọdịnaya nke sugars na carbohydrates soluble.
Schefflera arborescens (Hayata) Merr bụ osisi ịchọ mma na-adịgide adịgide nke ezinụlọ Araliaceae, nke China na Taiwan1.A na-akụkarị osisi a dị ka ahịhịa ụlọ, mana naanị otu osisi nwere ike itolite n'ọnọdụ ndị dị otú ahụ.Akwụkwọ ndị ahụ nwere site na 5 ruo 16 mpempe akwụkwọ, nke ọ bụla 10-20 cm2 ogologo.A na-ere Dwarf Schefflera n'ọtụtụ buru ibu kwa afọ, mana a naghị eji usoro ịkọ ugbo ọgbara ọhụrụ eme ihe.Ya mere, iji ndị na-ahụ maka mmepụta ihe ọkụkụ dị ka ngwá ọrụ nlekọta dị irè iji meziwanye ọganihu na mmepụta nke mmepụta ihe ọkụkụ na-achọ nlebara anya karị.Taa, ojiji nke ndị na-ahụ maka uto osisi abawanyela nke ukwuu3,4,5.Gibberellic acid bụ ihe na-achịkwa uto osisi nke nwere ike ịbawanye mkpụrụ osisi6.Otu n'ime mmetụta ya mara bụ mkpali nke uto ahịhịa, gụnyere azuokokoosisi na mgbọrọgwụ elongation na ụbara ebe akwukwo7.Mmetụta kachasị dị mkpa nke gibberellins bụ mmụba n'ịdị elu stem n'ihi ịgbatị internodes.Ịfesa gibberellins n'osisi dwarf nke na-enweghị ike ịmepụta gibberellins na-ebute mmụba nke stem elongation na ogologo osisi8.Ịfesa ifuru na akwụkwọ ya na gibberellic acid na mkpokọta 500 mg / l nwere ike ịbawanye elu osisi, ọnụọgụ, obosara na ogologo akwụkwọ9.A kọwo na Gibberellins na-akpali uto nke ahịhịa sara mbara dị iche iche10.A hụrụ elongation stem na Scots pine (Pinussylvestris) na ọcha spruce (Piceaglauca) mgbe a na-efesa akwụkwọ ya na gibberellic acid11.
Otu nnyocha nyochara mmetụta nke ndị na-ahụ maka uto osisi cytokinin atọ na nguzobe alaka mpụta na Lily officinalis.Emere nnwale n'oge mgbụsị akwụkwọ na udu mmiri iji mụọ mmetụta n'oge.Nsonaazụ gosiri na kinetin, benzyladenine na 2-prenyladenine emetụtaghị nguzobe nke alaka ndị ọzọ.Otú ọ dị, 500 ppm benzyladenine mere ka e guzobe alaka 12.2 na 8.2 n'oge mgbụsị akwụkwọ na nyocha mmiri, n'otu n'otu, ma e jiri ya tụnyere 4.9 na 3.9 alaka na osisi njikwa.Nnyocha egosiwo na ọgwụgwọ okpomọkụ dị irè karịa nke oyi12.Na nnwale ọzọ, Peace Lily var.A na-emeso osisi Tassone na 0, 250 na 500 ppm benzyladenine na ite 10 cm dayameta.Nsonaazụ gosiri na ọgwụgwọ ala nke ukwuu mụbara ọnụ ọgụgụ nke akwụkwọ ndị ọzọ ma e jiri ya tụnyere njikwa na benzyladenine na-agwọ osisi.A hụrụ akwụkwọ ọhụrụ ndị ọzọ ka izu anọ gachara ọgwụgwọ, a na-ahụkwa mmepụta akwụkwọ kachasị ka izu asatọ gachara ọgwụgwọ.N'ime izu 20 ka a gwọchara ya, ihe ọkụkụ ndị a na-eme n'ala nwere uru dị elu karịa osisi ndị a gwọchara ya13.A kọwo na benzyladenine na ntinye nke 20 mg / L nwere ike ịbawanye elu osisi na ọnụ ọgụgụ akwụkwọ na Croton 14. Na calla lilies, benzyladenine na ntinye nke 500 ppm mere ka ọnụ ọgụgụ nke alaka na-abawanye, ebe ọnụọgụgụ. nke alaka bụ nke kacha nta na njikwa15.Ebumnobi ọmụmụ ihe a bụ iji nyochaa ịgbasa foliar nke gibberellic acid na benzyladenine iji meziwanye uto nke Schefflera dwarfa, ihe ọkụkụ ịchọ mma.Ndị na-ahụ maka uto osisi a nwere ike inyere ndị na-akụ ahịa aka ịhazi mmepụta kwesịrị ekwesị kwa afọ.Enweghị ọmụmụ ihe emerela iji kwalite uto nke Liriodendron tulipifera.
Emere ọmụmụ ihe a n'ime ụlọ nyocha ihe ọkụkụ nke Mahadum Islamic Azad na Jiloft, Iran.Uniform mgbọrọgwụ transplants nke dwarf schefflera 25 ± 5 cm n'ịdị elu e njikere (propagated ọnwa isii tupu nnwale) na-akụ na akụwa.Ite ahụ bụ plastik, ojii, nke nwere dayameta nke 20 cm na ịdị elu nke 30 cm16.
Omenala n'ime ọmụmụ ihe a bụ ngwakọta nke peat, humus, aja a na-asachapụ na ahịhịa osikapa na nha 1: 1: 1: 1 (site na olu)16.Tinye akwa okwute n'ala ala ite maka igbapu mmiri.Nkezi okpomọkụ nke ehihie na abalị na griin haus na ngwụcha oge opupu ihe ubi na ọkọchị bụ 32±2°C na 28±2°C, n'otu n'otu.Iru mmiri dị ala na-eru> 70%.Jiri usoro misting maka ogbugba mmiri n'ubi.Ná nkezi, a na-agba osisi mmiri ugboro iri na abụọ n'ụbọchị.N'oge mgbụsị akwụkwọ na ọkọchị, oge ịgbara mmiri ọ bụla bụ nkeji 8, oge ịgbara mmiri bụ 1 awa.A na-akụ osisi n'otu aka ahụ ugboro anọ, 2, 4, 6 na 8 izu mgbe aghachara, yana ngwọta micronutrient (Ghoncheh Co., Iran) na ntinye nke 3 ppm ma gbanye ya na 100 ml nke ngwọta oge ọ bụla.Ihe ngwọta na-edozi ahụ nwere N 8 ppm, P 4 ppm, K 5 ppm na ihe ndị na-achọpụta Fe, Pb, Zn, Mn, Mo na B.
A kwadebere mkpokọta gibberellic acid atọ na onye na-ahụ maka uto osisi benzyladenine (nke a zụtara na Sigma) na 0, 100 na 200 mg/L wee fesa ya na buds osisi n'ime usoro atọ na etiti oge nke 15 days17.A na-eji Tween 20 (0.1%) (zụrụ na Sigma) na ngwọta iji mee ka ogologo ndụ ya dịkwuo ogologo na ọnụego nnabata.N'isi ụtụtụ, fesa homonụ ahụ na buds na akwụkwọ nke Liriodendron tulipifera site na iji sprayer.A na-efesa osisi na mmiri distilled.
Ogologo osisi, dayameta azuokokoosisi, mpaghara akwukwo, ọdịnaya chlorophyll, ọnụ ọgụgụ internodes, ogologo alaka nke abụọ, ọnụ ọgụgụ nke ngalaba nke abụọ, olu mgbọrọgwụ, ogologo mgbọrọgwụ, uka nke akwukwo, mgbọrọgwụ, stem na akọrọ ọhụrụ okwu, ọdịnaya nke photosynthetic pigments (chlorophyll). a, chlorophyll b) ngụkọta chlorophyll, carotenoids, ngụkọta pigmenti), mbenata shuga na carbohydrates soluble ka a tụrụ na ọgwụgwọ dị iche iche.
A tụrụ ọdịnaya chlorophyll nke akwụkwọ ndị na-eto eto ụbọchị 180 ka agbachara ya site na iji mita chlorophyll (Spad CL-01) site na 9:30 ruo 10 nke ụtụtụ (n'ihi ịdị ọhụrụ nke akwukwo).Na mgbakwunye, a tụrụ mpaghara akwukwo ụbọchị 180 ka ịgbasachara ya.Tulee akwụkwọ atọ si n'elu, etiti na ala nke azuokokoosisi sitere na ite ọ bụla.A na-eji akwụkwọ ndị a eme ihe dị ka ndebiri na akwụkwọ A4 ma bechapụ ihe na-esi na ya pụta.A tụrụkwa ịdị arọ na ebe elu nke otu mpempe akwụkwọ A4.Mgbe ahụ, a na-agbakọ akụkụ nke akwụkwọ stencil site na iji nha.Ọzọkwa, a na-ekpebi olu nke mgbọrọgwụ site na iji cylinder gụsịrị akwụkwọ.A na-atụle ibu akọrọ akwụkwọ, ibu akọrọ, mgbọrọgwụ nkụ, na mkpokọta akọrọ nke ihe nlele ọ bụla site na ihicha oven na 72 ° C maka awa 48.
A tụrụ ọdịnaya nke chlorophyll na carotenoids site na usoro Lichtenthaler18.Iji mee nke a, a na-akụ 0.1 g nke akwụkwọ ọhụrụ n'ime ngwa agha nwere 15 ml nke 80% acetone, na mgbe nzacha, a na-atụle njupụta anya ha site na iji spectrophotometer na ebili mmiri nke 663.2, 646.8 na 470 nm.Mepụta ngwaọrụ ahụ site na iji 80% acetone.Gbakọọ mkpokọta foto synthetic pigmenti na-eji usoro ndị a:
N'ime ha, Chl a, Chl b, Chl T na ụgbọ ala na-anọchi anya chlorophyll a, chlorophyll b, mkpokọta chlorophyll na carotenoids, n'otu n'otu.A na-egosipụta nsonaazụ ya na mg/ml osisi.
A tụrụ mbelata shuga site na iji usoro Somogy19.Iji mee nke a, a na-akụ 0.02 g nke ome osisi n'ime ngwa ngwa poselin na 10 ml nke mmiri distilled ma wụsa n'ime obere iko.Kpoo iko ahụ ka ọ bụrụ obụpde wee kpochaa ihe dị n'ime ya site na iji Whatman No. 1 akwụkwọ nzacha iji nweta ihe ọkụkụ.Nyefee 2 ml nke ọ bụla wepụ n'ime tube ule ma tinye 2 ml nke ọla kọpa sulfate ngwọta.Na-ekpuchi tube ule na owu owu wee kpoo ọkụ na mmiri ịsa ahụ na 100 ° C maka nkeji 20.N'oge a, a na-agbanwe Cu2+ ka ọ bụrụ Cu2O site na mbelata aldehyde monosaccharide na a na-ahụ agba salmon (terracotta) na ala nke tube ule.Mgbe tube ule ahụ dị jụụ, tinye 2 ml nke phosphomolybdic acid na agba anụnụ anụnụ ga-apụta.Kpochapụ tube ahụ nke ọma ruo mgbe agbasachara nke ọma na tube ahụ.Gụọ nnabata nke ngwọta na 600 nm site na iji spectrophotometer.
Gbakọọ mkpokọta nke ibelata shuga site na iji usoro ọkọlọtọ.Ekpebiri itinye uche nke carbohydrates soluble site na usoro Fales20.Iji mee nke a, a na-agwakọta 0.1 g nke pulitere na 2.5 ml nke 80% ethanol na 90 Celsius C maka 60 min (nkeji abụọ nke 30 min ọ bụla) iji wepụ carbohydrates soluble.Mgbe ahụ, a na-ekpocha ihe ndị ahụ ma wepụ mmanya na-aba n'anya.A na-agbaze ihe na-esi na ya pụta na 2.5 ml nke mmiri mmiri na-ekpo ọkụ.Wunye 200 ml nke ihe nlele ọ bụla n'ime tube ule wee gbakwunye 5 ml nke akara anthrone.A na-etinye ngwakọta ahụ na mmiri ịsa ahụ na 90 Celsius C maka 17 min, na mgbe ọ dị jụụ, a na-ekpebi ntinye ya na 625 nm.
Nnwale a bụ nnwale nke dabere na nhazi nke enweghị usoro nke nwere mmegharị anọ.A na-eji usoro PROC UNIVARIATE iji nyochaa ịdị mma nke nkesa data tupu nyocha nke iche.Nyocha ndekọ aha malitere site na nyocha ndekọ ọnụ ọgụgụ nkọwa iji ghọta ogo nke data raw anakọtara.Emebere mgbako iji mee ka ọ dị mfe na ịpịkọta nnukwu data iji mee ka ọ dị mfe ịtụgharị uche.N'ikpeazụ, e mere nyocha ndị dị mgbagwoju anya.Emere ule Duncan site na iji sọftụwia SPSS (ụdị 24; IBM Corporation, Armonk, NY, USA) iji gbakọọ square pụtara na njehie nnwale iji chọpụta ọdịiche dị n'etiti usoro data.Ejiri Duncan's multiple test (DMRT) chọpụta ọdịiche dị n'etiti ụzọ na ọkwa dị mkpa nke (0.05 ≤ p).A gbakọrọ ọnụ ọgụgụ mmekọrịta nke Pearson (r) site na iji ngwanrọ SPSS (ụdị 26; IBM Corp., Armonk, NY, USA) iji nyochaa njikọ dị n'etiti ụzọ abụọ dị iche iche.Tụkwasị na nke ahụ, a na-eme nyocha regression linear site na iji software SPSS (v.26) iji buru amụma ụkpụrụ nke mgbanwe afọ mbụ dabere na ụkpụrụ nke mgbanwe afọ nke abụọ.N'aka nke ọzọ, a na-eme nyocha nlọghachi azụ na p <0.01 iji chọpụta àgwà ndị na-emetụta akwụkwọ dwarf schefflera.A na-eme nyocha nke ụzọ iji chọpụta mmetụta kpọmkwem na nke na-apụtaghị ìhè nke àgwà ọ bụla na ihe nlereanya ahụ (dabere na njirimara ndị na-akọwa mgbanwe dị mma).Ngụkọta niile a dị n'elu (nkịtị nke nkesa data, ọnụọgụ mmekọrịta dị mfe, nkwụghachi azụ na nyocha ụzọ) mere site na iji ngwanrọ SPSS V.26.
Ụdị ihe ọkụkụ ndị a kpụrụ akpụ bụ n'ụzọ kwekọrọ na ụkpụrụ ụlọ ọrụ, mba na mba ụwa na iwu ụlọ nke Iran.
Tebụl 1 na-egosi ọnụ ọgụgụ nkọwa nke pụtara, ndakpọ ọkọlọtọ, kacha nta, kacha, oke, na phenotypic coefficient of variation (CV) maka àgwà dị iche iche.N'ime ọnụ ọgụgụ ndị a, CV na-enye ohere iji atụnyere njirimara n'ihi na ọ nweghị akụkụ.Mbelata shuga (40.39%), mgbọrọgwụ akọrọ (37.32%), mgbọrọgwụ ọhụrụ arọ (37.30%), sugar ka sugar ratio (30.20%) na mgbọrọgwụ olu (30%) bụ ndị kasị elu.yana ọdịnaya chlorophyll (9.88%).) na mpaghara akwukwo nwere ndepụta kachasị elu (11.77%) ma nwee uru CV kacha ala.Tebụl 1 na-egosi na mkpokọta mmiri dị arọ nwere oke kachasị elu.Agbanyeghị, njirimara a enweghị CV kachasị elu.Ya mere, ekwesịrị iji metrik na-enweghị akụkụ dị ka CV tulee mgbanwe àgwà.CV dị elu na-egosi nnukwu ọdịiche dị n'etiti ọgwụgwọ maka àgwà a.Nsonaazụ nke nnwale a gosipụtara nnukwu ọdịiche dị n'etiti ọgwụgwọ shuga dị ala na mgbọrọgwụ akọrọ, ịdị arọ mgbọrọgwụ ọhụrụ, oke carbohydrate-to-sugar, na njirimara olu mgbọrọgwụ.
Nsonaazụ ANOVA gosiri na atụnyere njikwa, foliar spraying na gibberellic acid na benzyladenine nwere mmetụta dị ukwuu n'ịdị elu osisi, ọnụ ọgụgụ nke akwụkwọ, mpaghara akwukwo, mgbọrọgwụ mgbọrọgwụ, ogologo mgbọrọgwụ, chlorophyll index, ọhụrụ arọ na akọrọ arọ.
Ntụle nke ụkpụrụ pụtara gosiri na ndị na-ahụ maka uto osisi nwere mmetụta dị ukwuu n'ịdị elu osisi na ọnụ ọgụgụ akwụkwọ.Ọgwụgwọ kachasị dị irè bụ gibberellic acid na ntinye nke 200 mg / l na gibberellic acid + benzyladenine na ntinye nke 200 mg / l.N'iji ya tụnyere njikwa, osisi dị elu na ọnụ ọgụgụ nke akwụkwọ mụbara site na 32.92 ugboro na 62.76 ugboro, n'otu n'otu (Table 2).
Mpaghara akwukwo abawanyela nke ukwuu na ụdị dị iche iche ma e jiri ya tụnyere njikwa, yana mmụba kachasị na 200 mg / l maka gibberellic acid, ruru 89.19 cm2.Nsonaazụ gosiri na mpaghara akwụkwọ mụbara nke ukwuu site n'ịba ụba nke ndị na-achịkwa uto (Table 2).
Ngwọta niile mụbara oke mgbọrọgwụ na ogologo oge ma e jiri ya tụnyere njikwa.Nchikota gibberellic acid + benzyladenine nwere mmetụta kachasị ukwuu, na-abawanye olu na ogologo mgbọrọgwụ site na ọkara ma e jiri ya tụnyere njikwa (Table 2).
A na-ahụ ụkpụrụ kachasị elu nke dayameta stem na ogologo internode na njikwa yana ọgwụgwọ gibberellic acid + benzyladenine 200 mg / l, n'otu n'otu.
Ndekọ chlorophyll mụbara n'ụdị niile ma e jiri ya tụnyere njikwa.A hụrụ uru kachasị elu nke àgwà a mgbe ejiri gibberellic acid + benzyladenine 200 mg / l, nke bụ 30.21% dị elu karịa njikwa (Table 2).
Nsonaazụ gosiri na ọgwụgwọ ahụ butere nnukwu ọdịiche dị na ọdịnaya pigmenti, mbelata shuga na carbohydrates soluble.
Ọgwụgwọ na gibberellic acid + benzyladenine butere oke ọdịnaya nke pigmenti photosynthetic.Ihe ịrịba ama a dị elu nke ukwuu na ụdịdị niile karịa na njikwa.
Nsonaazụ gosiri na ọgwụgwọ niile nwere ike ịbawanye ọdịnaya chlorophyll nke dwarf Schefflera.Otú ọ dị, a hụrụ uru kachasị elu nke àgwà a na ọgwụgwọ na gibberellic acid + benzyladenine, nke dị 36.95% dị elu karịa njikwa (Table 3).
Nsonaazụ maka chlorophyll b bụ kpamkpam yiri nsonaazụ chlorophyll a, naanị ihe dị iche bụ mmụba nke ọdịnaya chlorophyll b, nke dị 67.15% dị elu karịa na njikwa (Table 3).
Ọgwụgwọ ahụ butere mmụba dị ukwuu na mkpokọta chlorophyll ma e jiri ya tụnyere njikwa ahụ.Ọgwụgwọ na gibberellic acid 200 mg / l + benzyladenine 100 mg / l dugara uru kachasị elu nke àgwà a, nke bụ 50% dị elu karịa njikwa (Table 3).Dị ka nsonaazụ ya si kwuo, njikwa na ọgwụgwọ na benzyladinine na dose nke 100 mg / l mere ka ọnụ ọgụgụ kachasị elu nke àgwà a.Liriodendron tulipifera nwere uru kachasị elu nke carotenoids (Table 3).
Nsonaazụ gosiri na mgbe ejiri gibberellic acid gwọọ ya na mkpokọta 200 mg / L, ọdịnaya chlorophyll mụbara nke ukwuu ruo chlorophyll b (Fig 1).
Mmetụta gibberellic acid na benzyladenine na a/b Ch.Oke nke dwarf schefflera.(GA3: gibberellic acid na BA: benzyladenine).Otu akwụkwọ ozi dị na ọnụ ọgụgụ ọ bụla na-egosi na ọdịiche ahụ adịghị mkpa (P <0.01).
Mmetụta nke ọgwụgwọ ọ bụla na ịdị ọhụrụ na akọrọ nke osisi dwarf schefflera dị elu karịa nke njikwa.Gibberellic acid + benzyladenine na 200 mg/L bụ ọgwụgwọ kachasị dị irè, na-abawanye ibu ọhụrụ site na 138.45% ma e jiri ya tụnyere njikwa.N'iji ya tụnyere njikwa ahụ, ọgwụgwọ niile ma e wezụga 100 mg / L benzyladenine mụbara nnukwu ibu osisi akọrọ, na 200 mg/L gibberellic acid + benzyladenine rụpụtara uru kachasị elu maka àgwà a (Table 4).
Ọtụtụ n'ime ụdị dị iche iche dị iche na njikwa na nke a, yana ụkpụrụ kachasị elu bụ 100 na 200 mg / l benzyladenine na 200 mg / l gibberellic acid + benzyladenine (Fig 2).
Mmetụta nke gibberellic acid na benzyladenine na oke nke carbohydrates soluble na mbelata shuga na dwarf schefflera.(GA3: gibberellic acid na BA: benzyladenine).Otu mkpụrụedemede na ọnụ ọgụgụ nke ọ bụla na-egosi enweghị nnukwu ọdịiche (P <0.01).
Emere nyocha nlọghachi nke Stepwise iji chọpụta njirimara n'ezie wee ghọta nke ọma mmekọrịta dị n'etiti mgbanwe ndị nwere onwe na ọnụ ọgụgụ akwụkwọ na Liriodendron tulipifera.Mgbọrọgwụ olu bụ mgbanwe mbụ banyere n'ime ihe nlereanya ahụ, na-akọwa 44% nke mgbanwe ahụ.Ngbanwe na-esote bụ ibu mgbọrọgwụ ọhụrụ, mgbanwe abụọ a kọwara 63% nke mgbanwe dị na ọnụọgụ akwụkwọ (Table 5).
A na-eme nyocha nke ụzọ iji kọwakwuo nlọghachi nke nzọụkwụ (Table 6 na Figure 3).Mmetụta kachasị mma na ọnụọgụ akwụkwọ jikọtara ya na mgbọrọgwụ mgbọrọgwụ ọhụrụ (0.43), nke jikọtara ya na nọmba akwukwo (0.47).Nke a na-egosi na àgwà a na-emetụta mkpụrụ, ebe mmetụta ya na-apụtaghị ìhè site na àgwà ndị ọzọ bụ ihe na-adịghị mma, na enwere ike iji àgwà a dị ka nhọrọ nhọrọ na mmemme ozuzu maka dwarf schefflera.Mmetụta kpọmkwem nke mgbọrọgwụ mgbọrọgwụ adịghị mma (-0.67).Mmetụta nke àgwà a na ọnụ ọgụgụ nke epupụta bụ kpọmkwem, mmetụta na-apụtaghị ìhè adịghị mkpa.Nke a na-egosi na ibu mgbọrọgwụ olu, obere ọnụ ọgụgụ nke epupụta.
Onyonyo 4 na-egosi mgbanwe na nkwụghachi azụ azụ nke olu mgbọrọgwụ na ibelata shuga.Dabere na ọnụọgụ regression, nkeji ọ bụla na-agbanwe n'ogologo mgbọrọgwụ yana carbohydrates soluble pụtara na olu mgbọrọgwụ na ibelata shuga na-agbanwe site na nkeji 0.6019 na 0.311.
A na-egosi ọnụọgụ mmekọrịta nke Pearson nke àgwà uto na eserese 5. Nsonaazụ gosiri na ọnụ ọgụgụ nke akwụkwọ na elu osisi (0.379*) nwere njikọ na mkpa kachasị elu.
Maapụ ikpo ọkụ nke mmekọrịta dị n'etiti mgbanwe dị na ọnụọgụ njikọ ọnụ ọgụgụ uto.# Y Axis: 1-Index Ch., 2-Internode, 3-LAI, 4-N nke akwụkwọ, 5-ịdị elu nke ụkwụ, dayameta 6-stem.# N'akụkụ X axis: A - index H., B - anya n'etiti ọnụ ọnụ, C - LAY, D - N. nke akwukwo, E - elu nke trouser ụkwụ, F - dayameta nke azuokokoosisi.
The Pearson correlation coefficient maka mmiri mmiri metụtara àgwà e gosiri na Figure 6. Nsonaazụ na-egosi mmekọrịta dị n'etiti akwukwo mmiri arọ na n'elu ala akọrọ arọ (0.834 **), ngụkọta akọrọ (0.913**) na mgbọrọgwụ akọrọ arọ (0.562*). )..Mkpokọta akọrọ uka nwere njikọ dị mma kachasị elu na nke kachasị dị na oge ịse akọrọ (0.790**) na mgbọrọgwụ akọrọ (0.741**).
Maapụ ikpo ọkụ nke mmekọrịta dị n'etiti mgbanwe nha nha nha nha nha ọhụrụ.# Y axis: 1 - ịdị arọ nke akwụkwọ ọhụrụ, 2 - ịdị arọ nke mkpụrụ osisi ọhụrụ, 3 - ịdị arọ nke mgbọrọgwụ ọhụrụ, 4 - mkpokọta akwụkwọ ọhụrụ.# X-axis na-anọchi anya: A - ibu akwụkwọ ọhụrụ, B - ibu mkpụrụ osisi ọhụrụ, CW - ịdị arọ mgbọrọgwụ ọhụrụ, D - mkpokọta ibu ọhụrụ.
The Pearson correlation coefficients for akọrọ arọ metụtara àgwà e gosiri na Figure 7. Nsonaazụ gosiri na akwukwo akọrọ arọ, nwa osisi akọrọ arọ (0.848**) na ngụkọta akọrọ arọ (0.947**), nwa osisi akọrọ arọ (0.854**) na mkpokọta akọrọ (0.781**) nwere ụkpụrụ kachasị elu.njikọ dị mma na njikọ dị mkpa.
Maapụ okpomoku nke mmekọrịta dị n'etiti mgbanwe nha nha nha nha nhata.# Y axis na-anọchi anya: 1-akwukwo nkụ dị arọ, 2-bud arọ akọrọ, 3-mgbọrọgwụ arọ akọrọ, 4-ngụkọta arọ akọrọ.# X Axis: A-akwukwo akọrọ, ibu B-bud akọrọ, CW mgbọrọgwụ akọrọ, D-ngụkọta ịdị arọ akọrọ.
A na-egosi ọnụ ọgụgụ nke Pearson correlation coefficient of pigment properties na Figure 8. Nsonaazụ gosiri na chlorophyll a na chlorophyll b (0.716**), ngụkọta chlorophyll (0.968**) na mkpokọta pigmenti (0.954**);chlorophyll b na mkpokọta chlorophyll (0.868**) na mkpokọta pigmenti (0.851**);ngụkọta chlorophyll nwere njikọ kachasị mma yana njikọ dị mkpa yana ngụkọta pigmenti (0.984**).
Maapụ ikpo ọkụ nke mmekọrịta dị n'etiti ọnụọgụ ọnụọgụ chlorophyll.# Y axes: 1- Ọwa a, 2- Ọwa.b,3 – a/b ruru, 4 ọwa.Mkpokọta, 5-carotenoids, pigmentị mkpụrụ 6.# X-Akụkụ: A-Ch.aB-ch.b, C- a/b ruru, D-Ch.Mkpokọta ọdịnaya, E-carotenoids, F-mkpụrụ nke pigmenti.
Dwarf Schefflera bụ osisi na-ewu ewu n'ụwa nile, na uto na mmepe ya na-enweta nlebara anya dị ukwuu ugbu a.Ojiji nke ndị na-ahụ maka uto osisi butere nnukwu ọdịiche, yana ọgwụgwọ niile na-abawanye elu osisi ma e jiri ya tụnyere njikwa.Ọ bụ ezie na a na-ejikarị mkpụrụ ndụ ihe nketa na-achịkwa ịdị elu osisi, nchọpụta na-egosi na itinye ndị na-ahụ maka mmepụta ihe ọkụkụ nwere ike ịbawanye ma ọ bụ ibelata elu osisi.Ogologo osisi na ọnụ ọgụgụ akwụkwọ a na-eji gibberellic acid + benzyladenine 200 mg/L bụ nke kachasị elu, ruru 109 cm na 38.25, n'otu n'otu.N'ikwekọ na ọmụmụ ihe gara aga (SalehiSardoei et al.52) na Spathiphyllum23, mmụba yiri nke ahụ n'ịdị elu osisi n'ihi ọgwụgwọ gibberellic acid ka a hụrụ na marigolds potted, albus alba21, daylilies22, daylilies, agarwood na lilies udo.
Gibberellic acid (GA) na-arụ ọrụ dị mkpa na usoro physiological dị iche iche nke osisi.Ha na-akpali nkewa cell, cell elongation, stem elongation na size mmụba24.GA na-ebute nkewa cell na elongation na shoot apices na meristems25.Mgbanwe nke akwụkwọ gụnyekwara mbelata ogologo ịhịa aka n'ahụ, nha akwụkwọ nta, yana agba akwụkwọ ndụ akwụkwọ ndụ na-egbuke egbuke26.Nnyocha na-eji ihe mgbochi ma ọ bụ ihe mkpali egosila na ions calcium sitere na isi mmalite na-arụ ọrụ dị ka ndị ozi nke abụọ na ụzọ akara gibberellin na sorghum corolla27.HA na-abawanye ogologo osisi site n'ịkwalite njikọ nke enzymes nke na-eme ka ezumike mgbidi cell dị ka XET ma ọ bụ XTH, expansins na PME28.Nke a na-eme ka mkpụrụ ndụ na-abawanye ka mgbidi cell na-adaba na mmiri na-abanye na cell29.Ngwa nke GA7, GA3 na GA4 nwere ike ịbawanye elongation stem30,31.Gibberellic acid na-ebute elongation azuokokoosisi na osisi dwarf, na na osisi rosette ọ na-akwụsị ito eto na internode elongation32.Otú ọ dị, tupu ọmụmụ ogbo, azuokokoosisi ogologo na-abawanye na 4-5 ugboro mbụ ya elu33.Achịkọtara usoro GA biosynthesis n'ime osisi na foto 9.
GA biosynthesis na osisi na ọkwa nke endogenous bioactive GA, schematic nnọchiteanya nke osisi (n'aka nri) na GA biosynthesis (aka ekpe).Àkụ́ ndị ahụ bụ agba agba ka ha kwekọọ n'ụdị HA egosipụtara n'akụkụ ụzọ biosynthetic;ụta na-acha uhie uhie na-egosi mbelata ọkwa GC n'ihi nhazi mpaghara na akụkụ osisi, na akụ ojii na-egosi mmụba GC.N'ọtụtụ osisi, dị ka osikapa na anyụ, ọdịnaya GA dị elu na ala ma ọ bụ akụkụ ala nke akwukwo30.Ọzọkwa, ụfọdụ akụkọ na-egosi na ọdịnaya GA bioactive na-ebelata ka akwụkwọ na-agbatị site na base34.A maghị ọkwa gibberellin kpọmkwem n'okwu ndị a.
Ndị na-ahụ maka uto osisi na-emetụtakwa ọnụọgụ na mpaghara akwụkwọ.Nsonaazụ gosiri na ịba ụba nke onye na-ahụ maka uto ihe ọkụkụ mere ka mmụba dị ukwuu na mpaghara akwụkwọ na ọnụ ọgụgụ.A kọwo na Benzyladenine na-abawanye mmepụta nke akwukwo calla15.Dị ka nsonaazụ nke ọmụmụ a si dị, ọgwụgwọ niile na-emeziwanye mpaghara akwukwo na ọnụ ọgụgụ.Gibberellic acid + benzyladenine bụ ọgwụgwọ kachasị dị irè ma bute ọnụ ọgụgụ kachasị na mpaghara akwụkwọ.Mgbe ị na-eto dwarf schefflera n'ime ụlọ, enwere ike ịhụ mmụba na ọnụ ọgụgụ akwụkwọ.
Ọgwụ GA3 mụbara ogologo internode ma e jiri ya tụnyere benzyladenine (BA) ma ọ bụ enweghị ọgwụgwọ homonụ.Nsonaazụ a bụ ihe ezi uche dị na ya nyere ọrụ GA n'ịkwalite uto7.Ọganihu azuokokoosisi gosikwara ụdịrị nsonaazụ ahụ.Gibberellic acid mụbara ogologo nke azuokokoosisi mana belata dayameta ya.Agbanyeghị, ijikọ ngwa BA na GA3 mụbara ogologo steetị.Mmụba a dị elu ma e jiri ya tụnyere osisi ejiri BA ma ọ bụ enweghị homonụ.Ọ bụ ezie na gibberellic acid na cytokinins (CK) na-akwalite uto osisi, n'ọnọdụ ụfọdụ, ha na-enwe mmetụta dị iche na usoro dị iche iche35.Dịka ọmụmaatụ, a hụrụ mmekọrịta na-adịghị mma na mmụba nke ogologo hypocotyl na osisi ndị a na-emeso GA na BA36.N'aka nke ọzọ, BA budata ụba mgbọrọgwụ mgbọrọgwụ (Table 1).Mmụba mgbọrọgwụ n'ihi exogenous BA ka akọọrọ n'ọtụtụ osisi (dịka ụdị Dendrobium na Orchid) 37,38.
All hormonal ọgwụgwọ mụbara ọnụ ọgụgụ nke ọhụrụ epupụta.Mmụba eke na mpaghara akwukwo na ogologo azuokokoosisi site na ọgwụgwọ ngwakọta bụ ihe a na-achọsi ike n'ahịa.Ọnụ ọgụgụ nke akwụkwọ ọhụrụ bụ ihe dị mkpa na-egosi uto vegetative.Ejighi homonụ exogenous mee ihe na mmepụta azụmahịa nke Liriodendron tulipifera.Otú ọ dị, mmetụta na-akwalite uto nke GA na CK, na-etinye aka na nguzozi, nwere ike inye nghọta ọhụrụ iji meziwanye ihe ọkụkụ nke osisi a.N'ụzọ doro anya, mmetụta synergistic nke BA + GA3 ọgwụgwọ dị elu karịa nke GA ma ọ bụ BA na-elekọta naanị ya.Gibberellic acid na-abawanye ọnụ ọgụgụ nke akwụkwọ ọhụrụ.Ka akwụkwọ ọhụrụ na-etolite, ịba ụba nke akwụkwọ ọhụrụ nwere ike igbochi uto akwukwo39.A kọwala GA ka ọ kwalite mbufe sucrose site na sinks gaa na isi iyi40,41.Na mgbakwunye, ngwa nke GA na osisi perennial nwere ike ịkwalite uto nke akụkụ ahịhịa dị ka akwụkwọ na mgbọrọgwụ, si otú ahụ gbochie mgbanwe site na uto vegetative gaa na ịmụ nwa42.
Enwere ike ịkọwa mmetụta GA na ịba ụba ihe ọkụkụ site na mmụba nke photosynthesis n'ihi mmụba na mpaghara akwukwo43.A kọrọ na GA na-ebute mmụba na mpaghara akwukwo nke Maize34.Nsonaazụ gosiri na ịbawanye ụba BA na 200 mg / L nwere ike ịbawanye ogologo na ọnụ ọgụgụ nke alaka nke abụọ na olu mgbọrọgwụ.Gibberellic acid na-emetụta usoro cellular dị ka nkewa cell na-akpali akpali na elongation, si otú ahụ na-eme ka uto vegetative43.Tụkwasị na nke ahụ, HA na-agbasa mgbidi cell site na hydrolyzing starch n'ime shuga, si otú ahụ na-ebelata ikike mmiri nke cell, na-eme ka mmiri banye na cell na n'ikpeazụ na-eduga na cell elongation44.

 


Oge nzipu: Jun-11-2024