Nnyocha a na-egosi na ero Kosakonia oryziphila NP19 nke e ji mgbọrọgwụ rụọ, nke e kewapụrụ na mgbọrọgwụ osikapa, bụ ihe na-enye aka n'ịkwalite uto osisi na-egbu nje na ihe na-egbu nje maka njikwa mgbawa osikapa. E mere nnwale In vitro na akwụkwọ ọhụrụ nke Khao Dawk Mali 105 (KDML105) osikapa aromatic. Nsonaazụ gosiri na NP19 gbochiri uto nke fungal conidia osikapa. E gbochiri ọrịa fungal n'okpuru ọnọdụ ọgwụgwọ atọ dị iche iche: ịgba osikapa na NP19 na fungal conidia; ịgba akwụkwọ na NP19 na fungal conidia n'otu oge; na ịgba akwụkwọ na fungal conidia sochiri ya ọgwụgwọ NP19 awa 30 ka e mesịrị. Ọzọkwa, NP19 belatara uto hyphal fungal site na 9.9–53.4%. N'ime nnwale ite, NP19 mụbara ọrụ peroxidase (POD) na superoxide dismutase (SOD) site na 6.1% ruo 63.0% na 3.0% ruo 67.7%, n'otu n'otu, na-egosi usoro nchekwa osisi ka mma. Ma e jiri ya tụnyere njikwa NP19 na-enweghị ọrịa, osisi osikapa ndị nwere nje NP19 gosiri mmụba na ọdịnaya agba site na 0.3%–24.7%, ọnụọgụ nke mkpụrụ zuru oke kwa panicle site na 4.1%, mkpụrụ nke mkpụrụ zuru oke site na 26.3%, nha nha nke mkpụrụ site na 34.4%, na ọdịnaya nke ihe na-esi ísì ụtọ 2-acetyl-1-pyrroline (2AP) site na 10.1%. Na osisi osikapa ndị nwere nje NP19 na blast, mmụba ahụ bụ 0.2%–49.2%, 4.6%, 9.1%, 54.4%, na 7.5%, n'otu n'otu. Nnwale ndị e mere n'ubi gosiri na osisi osikapa ndị e ji NP19 mee ma ọ bụ gbaa ọgwụ n'ime ha gosiri mmụba nke ọnụọgụ mkpụrụ osisi zuru oke kwa paincle site na 15.1–27.2%, mkpụrụ ọka zuru oke site na 103.6–119.8%, na ọdịnaya 2AP site na 18.0–35.8%. Osisi osikapa ndị a gosikwara na ha na-arụ ọrụ SOD dị elu (6.9–29.5%) ma e jiri ya tụnyere osisi osikapa ndị gbawara agbawa nke a na-agbaghị ọgwụ NP19. Mgbe ọrịa gasịrị, itinye NP19 n'ahụ ya mere ka ọnyá ahụ ghara ịpụta ngwa ngwa. Ya mere, egosiri na K. oryziphila NP19 bụ uto osisi nwere ike ịkwalite ihe na-akpata ọrịa na ọgwụ nje maka njikwa nke mgbawa osikapa.
Agbanyeghị, ọtụtụ ihe na-emetụta irè nke ọgwụ fungicides, gụnyere nhazi, oge na ụzọ e si eji ya, oke ọrịa, irè nke usoro amụma ọrịa, na mpụta nke ụdị nje ndị na-eguzogide ọgwụ fungicides. Ọzọkwa, iji ọgwụ fungicides kemịkal nwere ike ibute nsị fọdụrụ na gburugburu ebe obibi ma tinye ihe egwu ahụike nye ndị na-eji ya.
N'ime nnwale ite ahụ, e tinyere mkpụrụ osikapa n'elu ma mịpụta ha dịka akọwara n'elu. E wee tinye K. oryziphila NP19 n'ime tray mkpụrụ osisi. E tinyere mkpụrụ osisi ndị ahụ n'ime ite ruo ụbọchị iri atọ ka mkpụrụ osikapa wee pụta. E wee tinye mkpụrụ osisi ndị ahụ n'ime ite. N'oge a na-etinye mkpụrụ osisi osikapa ahụ, e tinyere fatịlaịza iji kwadebe ha maka ọrịa nke ero na-akpata mgbawa osikapa na iji nwalee iguzogide ha.
N'ime nnwale ubi, e jiri usoro akọwara n'elu gwọọ mkpụrụ ndị gbawara agbawa nke nwere Aspergillus oryzae NP19 ma kewaa ha n'ìgwè abụọ: mkpụrụ ndị bu Aspergillus oryzae NP19 (RS) na mkpụrụ ndị na-enweghị ọrịa (US). A kụrụ mkpụrụ ndị gbawara agbawa n'ime tray nwere ala a gbawara agbawa (ngwakọta ala, akpụkpọ osikapa a gbara ọkụ, na nsị n'ogo 7:2:1 site na ibu) ma tinye ha n'ime akpa maka ụbọchị iri atọ.
Agbakwunyere oryziphila conidial suspension na osikapa R, mgbe awa iri atọ nke incubation gasịrị, etinyere 2 μl nke K. oryziphila NP19 n'otu ebe ahụ. A kpọchiri efere Petri niile na 25°C n'ọchịchịrị ruo awa iri atọ, wee kpọchie ha n'okpuru ìhè na-aga n'ihu. A kpọchiri otu ọ bụla ugboro atọ. Mgbe awa iri asaa na abụọ nke incubation gasịrị, a lere akụkụ osisi ahụ anya ma jiri igwe nyocha eletrọnịk. N'ụzọ dị mkpirikpi, etinyere akụkụ osisi ahụ na saline phosphate-buffered nke nwere 2.5% (v/v) glutaraldehyde ma kpọchie ha n'usoro ngwọta ethanol. E jiri carbon dioxide kpọọ ihe nlele ndị dị mkpa, wee kpuchie ha ọlaedo ma hụ ha n'okpuru igwe nyocha eletrọnịk maka nkeji iri na ise.
Oge ozi: Ọktoba-13-2025