Paclobutrasol na-akpalite triterpenoid biosynthesis site n'ịkwụsị onye na-ahazi transcriptional SlMYB na-adịghị mma na honeysuckle Japanese.

Nnukwu ero nwere ọtụtụ metabolites bara ụba na nke dị iche iche, a na-ewerekwa ya dị ka ihe bara uru bioresource. Phellinus igniarius bụ nnukwu ero a na-ejikarị eme ọgwụ na nri nri, mana nhazi ya na aha Latin ka na-arụ ụka. N'iji nyocha nhazi nhazi nke multigene, ndị nchọpụta ahụ kwadoro na Phellinus igniarius na ụdị ndị yiri ya bụ nke ọhụrụ genus ma guzobe genus Sanghuangporus. ero honeysuckle Sanghuangporus lonicericola bụ otu n'ime ụdị Sanghuangporus ama ama n'ụwa niile. Phellinus igniarius adọtala nlebara anya dị ukwuu n'ihi ọgwụ dị iche iche, gụnyere polysaccharides, polyphenols, terpenes, na flavonoids. Triterpenes bụ isi ihe na-arụ ọrụ ọgwụ na-arụ ọrụ nke ụdị a, na-egosipụta ọrụ antioxidant, antibacterial, na antitumor.
Triterpenoids nwere ikike dị ukwuu maka ngwa azụmahịa. N'ihi ụkọ nke anụ ọhịa Sanghuangporus dị n'okike, ịkwalite nrụpụta biosynthetic ya nke ọma na mkpụrụ dị oke mkpa. Ugbu a, enweela ọganihu n'ịkwalite mmepụta nke metabolites abụọ dị iche iche nke Sannghuangporus site n'iji kemịkalụ inducers na-achịkwa atumatu ịgba ụka. Ọmụmaatụ, polyunsaturated fatty acids, fungal elicitors11 na phytohormones (gụnyere methyl jasmonate na salicylic acid14) ka egosiri na-abawanye mmepụta triterpenoid na Sanghuangporus. Ndị na-achịkwa uto osisi(PGRs)nwere ike ịhazi biosynthesis nke metabolites nke abụọ na osisi. N'ime ọmụmụ ihe a, a nyochara PBZ, onye na-ahụ maka uto ihe ọkụkụ nke a na-ejikarị achịkwa uto osisi, mkpụrụ, àgwà na àgwà physiological. Karịsịa, iji PBZ nwere ike imetụta ụzọ terpenoid biosynthetic na osisi. Nchikota gibberellins na PBZ mụbara quinone methide triterpene (QT) ọdịnaya na Montevidia floribunda. Ihe mejupụtara nke ụzọ terpenoid nke mmanụ lavender gbanwere mgbe ọgwụgwọ na 400 ppm PBZ gasịrị. Otú ọ dị, ọ dịghị akụkọ banyere ngwa nke PBZ na mushrooms.
Na mgbakwunye na ọmụmụ ihe na-elekwasị anya na mmụba nke mmepụta triterpene, ụfọdụ ọmụmụ akọwawokwa usoro nhazi nke triterpene biosynthesis na Moriformis n'okpuru mmetụta nke ndị na-emepụta kemịkalụ. Ka ọ dị ugbu a, ọmụmụ ihe na-elekwasị anya na mgbanwe nke ọkwa nkwupụta nke mkpụrụ ndụ ihe nketa nke metụtara triterpene biosynthesis na ụzọ MVA, nke na-eduga n'ịbawanye mmepụta nke terpenoid.12,14 Otú ọ dị, ụzọ ndị dị n'okpuru mkpụrụ ndụ ihe nketa ndị a maara, karịsịa ihe odide ederede na-achịkwa okwu ha, na-anọgide na-edoghị anya na usoro nhazi nke triterpene biosynthesis.
N'ime ọmụmụ ihe a, a na-enyocha mmetụta dị iche iche nke ndị na-ahụ maka mmepụta ihe ọkụkụ (PGR) na mmepụta triterpene na uto mycelial n'oge fermentation nke honeysuckle (S. lonicericola). Mgbe nke ahụ gasịrị, a na-eji metabolomics na transcriptomics nyochaa nhazi triterpene na usoro okwu mkpụrụ ndụ ihe nketa na-etinye aka na triterpene biosynthesis n'oge ọgwụgwọ PBZ. RNA-sequencing na data bioinformatics achọpụtakwara ihe ebumnuche ederede MYB (SlMYB). Ọzọkwa, e mepụtara mutants iji kwado mmetụta nchịkwa nke SlMYB gene na triterpene biosynthesis ma chọpụta mkpụrụ ndụ ihe nketa nwere ike ime. Eji Electrophoretic mobility shift assays (EMSA) gosi na mmekọrịta nke protein SlMYB na ndị na-akwalite mkpụrụ ndụ ihe nketa SlMYB. Na nchịkọta, ebumnuche nke ọmụmụ a bụ iji kpalie triterpene biosynthesis site na iji PBZ ma chọpụta ihe MYB transcription factor (SlMYB) na-achịkwa kpọmkwem triterpene biosynthetic genes gụnyere MVD, IDI, na FDPS na S. lonicericola na nzaghachi na ntinye PBZ.
Ntinye nke ma IAA na PBZ budata mmụba triterpenoid na honeysuckle, mana mmetụta ntinye nke PBZ pụtara ìhè karịa. Ya mere, a chọpụtara na PBZ bụ onye na-emepụta ihe kacha mma na ntinye ntinye nke 100 mg / L, nke kwesịrị ka ọmụmụ ihe ọzọ.