Ero buru ibu nwere ọtụtụ ihe ndị na-arụ ọrụ n'ime ahụ, a na-ewerekwa ha dị ka ihe bara uru nye bioresources. Phellinus ignarius bụ ero buru ibu nke a na-ejikarị eme ihe maka ọgwụgwọ na nri, mana nhazi ya na aha Latin ka na-arụ ụka. Site na nyocha nhazi nke mkpụrụ ndụ ihe nketa dị iche iche, ndị nchọpụta kwadoro na Phellinus ignarius na ụdị ndị yiri ya so na ụdị ọhụrụ ma guzobe ụdị Sanghuangporus. Ero honeysuckle Sanghuangporus lonicericola bụ otu n'ime ụdị Sanghuangporus a ma ama n'ụwa niile. Phellinus ignarius adọtala mmasị dị ukwuu n'ihi ụdị ọgwụ ya dị iche iche, gụnyere polysaccharides, polyphenols, terpenes, na flavonoids. Triterpenes bụ ihe ndị bụ isi na-arụ ọrụ n'ihe gbasara ọgwụ nke ụdị a, na-egosipụta ọrụ antioxidant, antibacterial, na antitumor.
Triterpenoids nwere nnukwu ike maka ojiji azụmaahịa. N'ihi ụkọ akụrụngwa Sanghuangporus ọhịa n'okike, ime ka arụmọrụ na mmepụta ya dịkwuo mma dị oke mkpa. Ugbu a, enweela ọganihu n'ịkwalite mmepụta nke ọtụtụ metabolites nke Sanghuangporus nke abụọ site na iji ihe ndị na-akpali kemịkalụ iji chịkwaa usoro ịgbaze mmiri n'ime mmiri. Dịka ọmụmaatụ, egosila na polyunsaturated fatty acids, fungal ecliptors11 na phytohormones (gụnyere methyl jasmonate na salicylic acid14) na-eme ka mmepụta triterpenoid dịkwuo elu na Sanghuangporus. Ndị na-achịkwa uto osisi(PGRs)nwere ike ịchịkwa biosynthesis nke metabolites nke abụọ na osisi. N'ọmụmụ ihe a, a nyochachara PBZ, otu njikwa uto osisi nke a na-ejikarị achịkwa uto osisi, mmepụta, ịdị mma na àgwà anụ ahụ. Karịsịa, ojiji nke PBZ nwere ike imetụta ụzọ biosynthetic terpenoid na osisi. Njikọta nke gibberellins na PBZ mụbara ọdịnaya quinone methide triterpene (QT) na Montevidia floribunda. A gbanwere nhazi nke ụzọ terpenoid nke mmanụ lavender mgbe ejiri 400 ppm PBZ gwọọ ya. Agbanyeghị, enweghị akụkọ gbasara itinye PBZ na ero.
Na mgbakwunye na ọmụmụ ihe ndị lekwasịrị anya na mmụba nke mmepụta triterpene, ụfọdụ ọmụmụ ihe akọwapụtakwala usoro nhazi nke biosynthesis triterpene na Moriformis n'okpuru mmetụta nke ndị na-akpali kemịkalụ. Ugbu a, ọmụmụ ihe na-elekwasị anya na mgbanwe nke ọkwa ngosipụta nke mkpụrụ ndụ ihe nketa metụtara biosynthesis triterpene na ụzọ MVA, nke na-eduga na mmụba nke mmepụta terpenoid.12,14 Agbanyeghị, ụzọ ndị dị n'okpuru mkpụrụ ndụ ihe nketa ndị a maara, ọkachasị ihe ndị na-akọwapụta ihe na-achịkwa ngosipụta ha, ka edoghị anya na usoro nhazi nke biosynthesis triterpene na Moriformis.
N'ọmụmụ ihe a, a nyochachara mmetụta nke ọtụtụ ihe ndị na-achịkwa uto osisi (PGRs) nwere na mmepụta triterpene na uto mycelium n'oge ịgbara mmiri nke honeysuckle (S. lonicericola). Mgbe nke ahụ gasịrị, ejiri metabolomics na transcriptomics nyochaa nhazi triterpene na ụkpụrụ ngosipụta mkpụrụ ndụ ihe nketa metụtara na biosynthesis triterpene n'oge ọgwụgwọ PBZ. Data usoro RNA na bioinformatics chọpụtakwara ihe ntụgharị ebumnuche nke MYB (SlMYB). Ọzọkwa, e mepụtara mutants iji kwado mmetụta nchịkwa nke mkpụrụ ndụ ihe nketa SlMYB na biosynthesis triterpene ma chọpụta mkpụrụ ndụ ihe nketa nwere ike ịbụ ebumnuche. Ejiri nyocha mgbanwe mmegharị elektrọphoretic (EMSA) kwado mmekọrịta nke protein SlMYB na ndị na-akwalite mkpụrụ ndụ ihe nketa SlMYB. Na nchịkọta, ebumnuche nke ọmụmụ ihe a bụ iji PBZ kpalite biosynthesis triterpene ma chọpụta ihe MYB transcription factor (SlMYB) nke na-achịkwa mkpụrụ ndụ ihe nketa biosynthetic triterpene gụnyere MVD, IDI, na FDPS na S. lonicericola na nzaghachi maka PBZ induction.
Ntinye nke ma IAA na PBZ mụbara mmepụta triterpenoid nke ukwuu na honeysuckle, mana mmetụta mkpali nke PBZ pụtara ìhè karịa. Ya mere, achọpụtara na PBZ bụ ihe kacha mma na-akpali akpali na ntinye ọzọ nke 100 mg/L, nke kwesịrị ka a mụọ ya ọzọ.
Oge ozi: Ọgọst-19-2025