Phosphorylation na-eme ka onye na-achịkwa uto DELLA na Arabidopsis rụọ ọrụ site n'ịkwalite njikọ nke histone H2A na chromatin.

A na-echekwa protein DELLA dị ka ihe kacha mmandị na-achịkwa utonke na-arụ ọrụ dị mkpa n'ịchịkwa mmepe osisi dịka nzaghachi nye ihe ndị dị n'ime na gburugburu ebe obibi. DELLA na-arụ ọrụ dị ka onye na-achịkwa ihe ndekọ ma a na-ewe ya n'ọrụ ka ọ bụrụ onye na-akwalite ihe site na ijikọ na ihe ndị na-emepụta ihe (TFs) na histone H2A site na ngalaba GRAS ya. Ọmụmụ ihe ndị na-adịbeghị anya egosila na a na-achịkwa nkwụsi ike DELLA site na usoro abụọ: polyubiquitination nke phytohormone gibberellin kpatara, nke na-eduga na mbibi ngwa ngwa ya, na njikọta nke obere ihe ndị yiri ubiquitin (SUMO) iji mee ka mkpokọta ya dịkwuo elu. Na mgbakwunye, a na-achịkwa ọrụ DELLA site na glycosylations abụọ dị iche iche: mmekọrịta DELLA-TF na-eme ka mmekọrịta DELLA-TF ka mma mana mgbanwe N-acetylglucosamine (O-GlcNAc) na-egbochi ya. Agbanyeghị, ọrụ nke DELLA phosphorylation ka edoghị anya, ebe ọmụmụ ihe ndị gara aga egosila nsonaazụ na-emegiderịta onwe ha, site na ndị na-egosi na phosphorylation na-akwalite ma ọ bụ na-ebelata mmebi DELLA gaa na ndị ọzọ na-egosi na phosphorylation anaghị emetụta nkwụsi ike ya. N'ebe a, anyị na-achọpụta ebe phosphorylation na REPRESSORga1-3(RGA, AtDELLA) e mere ka ọ dị ọcha site na Arabidopsis thaliana site na nyocha nke oke spectrometry ma gosi na phosphorylation nke peptides RGA abụọ na mpaghara PolyS na PolyS/T na-akwalite njikọ H2A na ọrụ RGA ka mma. Njikọ nke RGA na ndị na-akwalite ebumnuche. Nke pụtara ìhè bụ na phosphorylation anaghị emetụta mmekọrịta RGA-TF ma ọ bụ nkwụsi ike RGA. Ọmụmụ ihe anyị na-ekpughe usoro molekul nke phosphorylation si ebute ọrụ DELLA.
Iji kọwaa ọrụ nke phosphorylation n'ịchịkwa ọrụ DELLA, ọ dị mkpa ịchọpụta ebe DELLA phosphorylation dị na vivo ma mee nyocha ọrụ na osisi. Site na nhicha nke ihe ndị sitere na osisi na-esote nyocha MS/MS, anyị chọpụtara ọtụtụ phosphosites na RGA. N'ọnọdụ enweghị GA, phosphorylation RHA na-abawanye, mana phosphorylation anaghị emetụta nkwụsi ike ya. Ihe dị mkpa bụ na nyocha co-IP na ChIP-qPCR kpughere na phosphorylation na mpaghara PolyS/T nke RGA na-akwalite mmekọrịta ya na H2A na njikọ ya na ndị na-akwalite ebumnuche, na-ekpughe usoro nke phosphorylation si ebute ọrụ RGA.
A na-akpọ RGA ka ọ gaa lekwasị anya na chromatin site na mmekọrịta nke ngalaba LHR1 na TF wee jikọọ na H2A site na mpaghara PolyS/T ya na ngalaba PFYRE, na-emepụta mgbagwoju anya H2A-RGA-TF iji mee ka RGA kwụsie ike. Phosphorylation nke Pep 2 na mpaghara PolyS/T n'etiti ngalaba DELLA na ngalaba GRAS site na kinase a na-amaghị ama na-eme ka njikọ RGA-H2A dịkwuo mma. Protein mutant rgam2A na-ewepụ phosphorylation RGA ma na-anabata nhazi protein dị iche iji gbochie njikọ H2A. Nke a na-ebute mmebi nke mmekọrịta TF-rgam2A na-adịghị adịte aka na nkewa nke rgam2A site na chromatin ebumnuche. Ọnụọgụ a na-egosi naanị njide ntụgharị ederede nke RGA na-akwado. Enwere ike ịkọwa ụkpụrụ yiri nke ahụ maka mkpali ntụgharị ederede nke RGA na-akwalite, belụsọ na mgbagwoju anya H2A-RGA-TF ga-akwalite ntụgharị mkpụrụ ndụ ihe nketa ebumnuche na dephosphorylation nke rgam2A ga-ebelata ntụgharị ederede. Edegharịrị eserese site na Huang et al.21.
E jiri Excel nyochaa data ọnụọgụgụ niile, e jikwa ule t nke ụmụ akwụkwọ chọpụta ọdịiche dị mkpa. E jirighị usoro ọnụọgụgụ chọpụta nha ihe nlele ahụ tupu oge eruo. E wepụghị data ọ bụla na nyocha ahụ; a naghị enyocha nnwale ahụ; ndị nchọpụta ahụ elegharaghị nkesa data anya n'oge nnwale ahụ na nyocha nke nsonaazụ ya. E gosipụtara nha ihe nlele ahụ na akụkọ ihe mere eme na faịlụ data isi mmalite.
Maka ozi ndị ọzọ gbasara nhazi ọmụmụ ihe a, lee Nchịkọta Akụkọ Nchịkọta Ihe Okike nke metụtara akụkọ a.
E tinyela data proteomics nke oke spectrometry na ProteomeXchange site na ebe nchekwa PRIDE66 nke nwere njirimara data PXD046004. E gosipụtara data ndị ọzọ niile e nwetara n'oge ọmụmụ ihe a na Ozi Mgbakwunye, Faịlụ Data Mgbakwunye, na Faịlụ Data Raw. E nyere data isi mmalite maka akụkọ a.