A na-echekwa protein DELLA nna ukwundị na-achịkwa utonke na-ekere òkè dị mkpa n'ịchịkwa mmepụta ihe ọkụkụ na nzaghachi nke ime na gburugburu ebe obibi. DELLA na-arụ ọrụ dị ka onye na-ahụ maka ntụgharị ederede ma na-eweghachite ya iji lekwasị ndị na-akwalite ya anya site na ijikọ ihe odide ederede (TFs) na histone H2A site na ngalaba GRAS ya. Nnyocha e mere n'oge na-adịbeghị anya egosila na a na-edozi nkwụsi ike nke DELLA site na nsụgharị abụọ site na usoro abụọ: polyubiquitination nke phytohormone gibberellin na-akpata, nke na-eduga ná mmebi ngwa ngwa ya, na njikọ nke obere ubiquitin-like modifiers (SUMO) iji mee ka nchịkọta ya dịkwuo elu. Na mgbakwunye, a na-ahazi ọrụ DELLA nke ukwuu site na glycosylations abụọ dị iche iche: mmekọrịta DELLA-TF na-eme ka O-fucosylation na-eme ka ọ dịkwuo mma mana mgbanwe N-acetylglucosamine (O-GlcNAc) jikọtara O-njikọ. Otú ọ dị, ọrụ nke DELLA phosphorylation nọgidere na-edoghị anya, dịka nchọpụta ndị gara aga egosiwo ihe ndị na-emegiderịta onwe ha, sitere na ndị na-egosi na phosphorylation na-akwalite ma ọ bụ na-ebelata mmebi DELLA na ndị ọzọ na-egosi na phosphorylation adịghị emetụta nkwụsi ike ya. N'ebe a, anyị na-achọpụta saịtị phosphorylation na REPRESSORga1-3(RGA, AtDELLA) sachara site na Arabidopsis thaliana site na nyocha nke uka spectrometry wee gosi na phosphorylation nke peptides RGA abụọ na mpaghara PolyS na PolyS/T na-akwalite njikọ H2A na nkwalite ọrụ RGA. Njikọ nke RGA na ndị na-akwalite ebumnuche. N'ụzọ doro anya, phosphorylation anaghị emetụta mmekọrịta RGA-TF ma ọ bụ nkwụsi ike RGA. Ọmụmụ ihe anyị na-ekpughe usoro molekụla nke phosphorylation ji ebute ọrụ DELLA.
Iji kọwapụta ọrụ phosphorylation n'ịhazi ọrụ DELLA, ọ dị oke mkpa ịchọpụta saịtị DELLA phosphorylation na vivo ma mee nyocha ọrụ na osisi. Site na ịdị ọcha nke ihe ọkụkụ na-esochi nyocha MS/MS, anyị chọpụtara ọtụtụ phosphosites na RGA. N'okpuru ọnọdụ nke ụkọ GA, RHA phosphorylation na-abawanye, mana phosphorylation anaghị emetụta nkwụsi ike ya. N'ụzọ dị mkpa, nyocha co-IP na ChIP-qPCR gosipụtara na phosphorylation na mpaghara PolyS / T nke RGA na-akwalite mmekọrịta ya na H2A na njikọ ya na ndị na-akwalite ebumnuche, na-ekpughe usoro nke phosphorylation na-ebute ọrụ RGA.
A na-eweghachi RGA ka o lekwasịrị anya chromatin site na mmekọrịta nke subdomain LHR1 na TF wee jikọta na H2A site na mpaghara PolyS/T ya na mpaghara PFYRE, na-eme ka mgbagwoju H2A-RGA-TF kwụsie ike RGA. Phosphorylation nke Pep 2 na mpaghara PolyS/T n'etiti ngalaba DELLA na ngalaba GRAS site na kinase na-amaghị ama na-eme ka njikọ RGA-H2A dịkwuo elu. Protein mutant rgam2A na-ewepụ RGA phosphorylation ma nakwere ngbanwe protein dị iche iji gbochie njikọ H2A. Nke a na-ebute nkwụsịtụ nke mmekọrịta TF-rgam2A na-agafe agafe na nkewa nke rgam2A site na chromatin lekwasịrị anya. Ọnụọgụ a na-egosi naanị mkpagharị ederede na RGA. Enwere ike kọwapụta ụkpụrụ yiri nke ahụ maka ịgbalite ntụgharị ntụgharị mgbasa ozi RGA, belụsọ na mgbagwoju H2A-RGA-TF ga-akwalite ntụgharị mkpụrụ ndụ ebumnuche yana dephosphorylation nke rgam2A ga-ebelata ndegharị. Ọnụego mgbanwe nke Huang et al.21.
A na-enyocha ọnụọgụ ọnụọgụ niile site na iji Excel, ma ekpebiela nnukwu ọdịiche site na iji ule Student's t. Ejighị ụzọ ndekọ ọnụ ọgụgụ buru ụzọ chọpụta nha nlele ahụ. Enweghị data ewepụrụ na nyocha; nnwale ahụ enweghị usoro; ndị nchọpụta ahụ kpuru ìsì na nkesa nke data n'oge nnwale na nyocha nke nsonaazụ. A na-egosipụta nha nlele ahụ na akụkọ akụkọ ọnụọgụ na faịlụ data isi mmalite.
Maka ozi ndị ọzọ gbasara nhazi ọmụmụ, lee akwụkwọ akụkọ akụkọ Pọtụfoliyo eke jikọtara ya na isiokwu a.
Enyerela data proteomics mass spectrometry na ProteomeXchange consortium site na ebe nchekwa ndị mmekọ PRIDE66 nwere njirimara dataset PXD046004. A na-egosi data ndị ọzọ niile enwetara n'oge ọmụmụ ihe na Ozi Mgbakwụnye, Faịlụ Data Mgbakwụnye, na Faịlụ Data Raw. Enyere data isi mmalite maka edemede a.
Oge nzipu: Nov-08-2024