A na-echekwa protein DELLAndị na-achịkwa utonke na-ekere òkè dị mkpa na mmepe osisi na nzaghachi nke n'ime na n'èzí mgbaàmà. Dị ka ndị na-ahụ maka ntụgharị ederede, DELLA na-ejikọta na ihe ntụgharị ederede (TFs) na histone H2A site na ngalaba GRAS ha ma na-eweghachite ha ka ha mee ihe na ndị nkwalite. Nnyocha e mere n'oge na-adịbeghị anya egosila na a na-ahazi nkwụsi ike nke DELLA site na usoro abụọ: polyubiquitation nke hormone osisi kpatara.gibberellin, nke na-eduga na mbibi ngwa ngwa ha, na njikọ na obere ubiquitin-like modifier (SUMO), nke na-eme ka nchịkọta ha dịkwuo elu. Ọzọkwa, a na-achịkwa ọrụ DELLA site na usoro glycosylation abụọ dị iche iche: O-fucosylation na-eme ka mmekọrịta DELLA-TF dịkwuo mma, ebe N-acetylglucosamine (O-GlcNAc) jikọtara O-njikọ na-egbochi mmekọrịta DELLA-TF. Otú ọ dị, ọrụ nke DELLA phosphorylation adịghị edozi dị ka nchọpụta ndị gara aga egosiwo ihe ndị na-emegiderịta onwe ha, na ụfọdụ na-atụ aro na phosphorylation na-akwalite ma ọ bụ mebie mmebi DELLA na ndị ọzọ na-atụ aro na phosphorylation adịghị emetụta nkwụsi ike ha. N'ebe a, anyị na-achọpụta saịtị phosphorylation na GA1-3 repressor (RGA), AtDELLA, dị ọcha site na Arabidopsis thaliana site na nnukwu spectrometry ma gosi na phosphorylation nke abụọ RGA peptides na mpaghara PolyS na PolyS / T na-eme ka ọrụ RGA dịkwuo mma site n'ịkwalite njikọ H2A na njikọ RGA na ndị na-ebuli elu. N'ụzọ doro anya, phosphorylation emetụtaghị mmekọrịta RGA-TF ma ọ bụ nkwụsi ike RGA. Ọmụmụ ihe anyị na-ekpughe usoro molekụla nke phosphorylation na-ebute ọrụ DELLA.
Ntụle spectrometric anyị nke ukwuu gosiri na ma Pep1 na Pep2 nwere nnukwu phosphorylated na RGA na GA-deficient Ga1 ndabere. Na mgbakwunye na ọmụmụ ihe a, ọmụmụ ihe ọmụmụ phosphoproteomic ekpugherekwa Pep1 phosphorylation na RGA, ọ bụ ezie na amụbeghị ọrụ ya53,54,55. N'ụzọ dị iche, Pep2 phosphorylation ka akọpụtabeghị na mbụ ebe ọ bụ na a ga-achọpụta peptide a naanị site na iji transgene RGAGKG. Ọ bụ ezie na m1A mutation, nke kagburu Pep1 phosphorylation, na-ebelata obere ọrụ RGA na planta, ọ nwere mmetụta mgbakwunye mgbe ejikọtara ya na m2A na mbenata ọrụ RGA (Ngbakwunye 6). N'ụzọ dị mkpa, Pep1 phosphorylation na-ebelata nke ukwuu na GA-enhanced sly1 mutant tụnyere ga1, na-atụ aro na GA na-akwalite RGA dephosphorylation, na-ebelata ọrụ ya. Usoro nke GA na-egbochi phosphorylation RGA chọrọ nyocha ọzọ. Otu ihe nwere ike ime bụ na a na-enweta nke a site na nhazi nke protein kinase na-amaghị ama. Ọ bụ ezie na ọmụmụ egosila na nkwupụta nke CK1 protein kinase EL1 na-achịkwa GA na osikapa41, nsonaazụ anyị na-egosi na mgbanwe dị elu nke Arabidopsis EL1 homologue (AEL1-4) adịghị ebelata RGA phosphorylation. N'ikwekọ na nsonaazụ anyị, ihe ọmụmụ phosphoproteomic na nso nso a site na iji Arabidopsis AEL overexpressing line na ael triple mutant achọpụtaghị protein ọ bụla DELLA dị ka mkpụrụ nke kinases56. Mgbe anyị kwadoro ihe odide ahụ, a kọrọ na GSK3, gene encoding a GSK3/SHAGGY-like kinase in wheat (Triticum aestivum), nwere ike phosphorylate DELLA (Rht-B1b)57, ọ bụ ezie na phosphorylation nke Rht-B1b site GSK3 ekwenyebeghị na osisi. In vitro enzymatic mmeghachi omume n'ihu GSK3 sochiri mass spectrometry analysis kpughere atọ phosphorylation saịtị dị n'etiti DELLA na GRAS ngalaba nke Rht-B1b (Mgbakwunye Fig. 3). Serine na alanine nnọchi na saịtị phosphorylation atọ niile butere mbelata ọrụ Rht-B1b na ọka wit transgenic, kwekọrọ na nchoputa anyị na nnọchi alanine na Pep2 RGA belatara ọrụ RGA. Agbanyeghị, nyocha mmebi protein na vitro gosipụtara n'ihu na phosphorylation nwekwara ike ime ka Rht-B1b57 kwụsie ike. Nke a dị iche na nsonaazụ anyị na-egosi na nnọchi alanine na Pep2 RGA anaghị agbanwe nkwụsi ike ya na planta. GSK3 na ọka wit bụ ortholog nke brassinosteroid-insensitive protein 2 (BIN2) na Arabidopsis 57. BIN2 bụ onye na-achịkwa ihe na-adịghị mma nke akara ngosi BR, na BR na-eme ka ụzọ mgbaàmà ya na-eme ka ọ bụrụ BIN2 mmebi 58. Anyị gosiri na ọgwụgwọ BR adịghị ebelata RGA nkwụsi ike 59 ma ọ bụ phosppleidation na ọkwa RGA, na-atụ aro ọkwa nke Arab RGA. O yighị ka BIN2 ga-eme ka phosphorylate.
A na-enyocha ọnụọgụ ọnụọgụ niile site na iji Excel, ma ekpebiela nnukwu ọdịiche site na iji t-ule nwata akwụkwọ. Ọ nweghị ụzọ ndekọ aha eji ebute ụzọ chọpụta nha nlele. Enweghị data ewepụrụ na nyocha; nnwale ahụ enweghị usoro; na ndị na-eme nchọpụta maara banyere oke n'oge nnwale na ntule nsonaazụ. A na-enye nha nlele na akụkọ ifo na na faịlụ data raw.
Oge nzipu: Eprel-15-2025