Ọrụ nke Uniconazole

       Uniconazolebụ triazoleihe na-achịkwa uto osisinke a na-ejikarị achịkwa ogologo osisi ma gbochie uto nke mkpụrụ osisi. Agbanyeghị, usoro molekul nke uniconazole ji egbochi ogologo hypocotyl nke mkpụrụ osisi ka edoghị anya, enwere naanị ọmụmụ ole na ole na-ejikọta data transcriptome na metabolome iji nyochaa usoro nke ogologo hypocotyl. N'ebe a, anyị hụrụ na uniconazole gbochiri ogologo hypocotyl nke ukwuu na mkpụrụ osisi kabeeji okooko osisi China. N'ụzọ na-adọrọ mmasị, dabere na nyocha transcriptome na metabolome jikọtara ọnụ, anyị chọpụtara na uniconazole metụtara ụzọ "phenylpropanoid biosynthesis" nke ukwuu. N'ụzọ a, naanị otu mkpụrụ ndụ ihe nketa nke ezinụlọ mkpụrụ ndụ ihe nketa enzyme, BrPAL4, nke metụtara biosynthesis lignin, dara nke ukwuu. Na mgbakwunye, nyocha yist otu-hybrid na abụọ-hybrid gosiri na BrbZIP39 nwere ike ijikọ ozugbo na mpaghara nkwalite nke BrPAL4 ma mee ka ntụgharị ya rụọ ọrụ. Sistemụ mmechi mkpụrụ ndụ ihe nketa nke nje virus kpatara gosikwara na BrbZIP39 nwere ike ịchịkwa ogologo hypocotyl nke kabeeji China na njikọ hypocotyl lignin nke ọma. Nsonaazụ nke ọmụmụ a na-enye nghọta ọhụrụ na usoro nhazi molekul nke cloconazole na-egbochi ogologo hypocotyl nke kabeeji China. E kwadoro ya maka oge mbụ na cloconazole belatara ọdịnaya lignin site na igbochi njikọ phenylpropanoid nke modulu BrbZIP39-BrPAL4 kwadoro, si otú a na-eduga na hypocotyl dwarfing na mkpụrụ osisi kabeeji China.

Kabeeji ndị China (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) so na ụdị Brassica ma bụrụkwa akwụkwọ nri a ma ama kwa afọ a na-akọkarị n'obodo m (Wang et al., 2022; Yue et al., 2022). N'afọ ndị na-adịbeghị anya, oke mmepụta nke kọlịflawa ndị China anọgidewo na-agbasa, usoro ịkọ ihe agbanweela site na ịgha mkpụrụ ozugbo ọdịnala gaa na ọdịbendị na ntụgharị mkpụrụ siri ike. Agbanyeghị, n'usoro nke ịkọ mkpụrụ na ntụgharị mkpụrụ siri ike, uto hypocotyl dị oke elu na-emepụta mkpụrụ osisi ụkwụ, na-ebute àgwà mkpụrụ osisi na-adịghị mma. Ya mere, ijikwa uto hypocotyl dị oke njọ bụ nsogbu dị mkpa na ọdịbendị mkpụrụ osisi siri ike na ntụgharị kabeeji ndị China. Ugbu a, enwere ọmụmụ ole na ole na-ejikọta data transcriptomics na metabolomics iji nyochaa usoro nke ogologo oge hypocotyl. A kabeghị amụ usoro molekul nke chlorantazole ji achịkwa mmụba hypocotyl na kabeeji ndị China. Ebumnuche anyị bụ ịchọpụta mkpụrụ ndụ ihe nketa na ụzọ molekul ndị na-aza maka hypocotyl nke uniconazole kpatara na kabeeji ndị China. Site na iji nyocha transcriptome na metabolomic, yana nyocha yist otu-hybrid, nyocha luciferase abụọ, na nnwale mkpụrụ ndụ ihe nketa nke nje kpatara (VIGS), anyị chọpụtara na uniconazole nwere ike ịkpalite hypocotyl dwarfing na kabeeji China site na igbochi biosynthesis lignin na mkpụrụ osisi kabeeji China. Nsonaazụ anyị na-enye nghọta ọhụrụ na usoro nchịkwa molekul nke uniconazole na-egbochi ogologo hypocotyl na kabeeji China site na igbochi biosynthesis phenylpropanoid nke modulu BrbZIP39–BrPAL4 kwadoro. Nsonaazụ ndị a nwere ike inwe mmetụta dị mkpa bara uru maka imeziwanye ogo nke mkpụrụ osisi azụmaahịa na inye aka na ijide n'aka na mkpụrụ na ịdị mma nke akwụkwọ nri.
E tinyere BrbZIP39 ORF zuru oke n'ime pGreenll 62-SK iji mepụta ihe na-eme ihe, e tinyekwara akụkụ ihe na-eme ka BrPAL4 dịkwuo na mkpụrụ ndụ ihe nketa nke pGreenll 0800 luciferase (LUC) iji mepụta mkpụrụ ndụ ihe nketa nke onye na-eme ihe na onye na-ede akụkọ. E tinyere ihe na-eme ihe na ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe na-eme ka ihe ghara ịdị irè ka ọ bụrụ akwụkwọ ụtaba (Nicotiana benthamiana).
Iji mee ka mmekọrịta dị n'etiti metabolites na mkpụrụ ndụ ihe nketa doo anya, anyị mere nyocha njikọta nke metabolome na transcriptome. Nyocha mmụba ụzọ KEGG gosiri na e jikọtara DEG na DAM n'ụzọ 33 nke KEGG (Foto 5A). N'ime ha, ụzọ "phenylpropanoid biosynthesis" bụ nke kacha baa ọgaranya; ụzọ "photosynthesis carbon fixation", ụzọ "flavonoid biosynthesis", ụzọ "pentose-glucuronic acid interconversion", ụzọ "tryptophan metabolism", na ụzọ "starch-sucrose metabolism" mekwara ka ọ baa ọgaranya nke ukwuu. Maapụ nchịkọta okpomọkụ (Foto 5B) gosiri na e kewara DAM ndị metụtara DEG n'ọtụtụ ụdị, n'ime ha bụ flavonoids bụ ụdị kachasị ukwuu, na-egosi na ụzọ "phenylpropanoid biosynthesis" rụrụ ọrụ dị mkpa na hypocotyl dwarfism.
Ndị dere akwụkwọ ahụ kwupụtara na emere nnyocha ahụ n'enweghị mmekọrịta azụmaahịa ma ọ bụ ego ọ bụla nke enwere ike ịkọwa dị ka esemokwu mmasị nwere ike ime.
Echiche niile e depụtara n'isiokwu a bụ nke onye dere ya, ọ bụghịkwa echiche nke ndị otu, ndị mbipụta akwụkwọ, ndị nchịkọta akụkọ, ma ọ bụ ndị nyocha. Ngwaahịa ọ bụla a tụlere n'isiokwu a ma ọ bụ nkwupụta nke ndị nrụpụta ha kwuru abụghị nke onye mbipụta akwụkwọ kwadoro ma ọ bụ kwadoro.