Uniconazolebụ triazoleonye na-ahụ maka uto osisinke a na-ejikarị eme ihe iji chịkwaa ịdị elu osisi ma gbochie itolite mkpụrụ osisi. Otú ọ dị, usoro molekụla nke uniconazole na-egbochi seedling hypocotyl elongation ka amabeghị, na e nwere nanị ole na ole ọmụmụ na-ejikọta transcriptome na metabolome data iji nyochaa usoro nke hypocotyl elongation. N'ebe a, anyị chọpụtara na uniconazole gbochiri hypocotyl elongation nke ukwuu na osisi kabeeji nke China. N'ụzọ na-akpali mmasị, dabere na nchịkọta transcriptome na metabolome jikọtara ọnụ, anyị chọpụtara na uniconazole metụtara nke ọma ụzọ "phenylpropanoid biosynthesis". N'ụzọ a, ọ bụ naanị otu mkpụrụ ndụ ihe nketa nke ezinụlọ enzyme regulatory gene, BrPAL4, nke na-etinye aka na lignin biosynthesis, ka ewedara nke ukwuu. Na mgbakwunye, yist otu ngwakọ na ngwakọ abụọ gosipụtara na BrbZIP39 nwere ike jikọọ ozugbo na mpaghara nkwalite BrPAL4 wee rụọ ọrụ ntụgharị ya. Sistemụ ịgbachitere mkpụrụ ndụ ihe nketa nke nje butere n'ihu gosipụtara na BrbZIP39 nwere ike ịhazigharị hypocotyl elongation nke kabeeji China na njikọ hypocotyl lignin. Nsonaazụ nke ọmụmụ a na-enye nghọta ọhụrụ n'ime usoro nchịkwa ihe omimi nke cloconazole na igbochi hypocotyl elongation nke kabeeji China. Ekwenyere na nke mbụ cloconazole belatara ọdịnaya lignin site na igbochi phenylpropanoid synthesis nke BrbZIP39-BrPAL4 modul gbaziri, si otú ahụ na-eduga na hypocotyl dwarfing na Chinese kabeeji seedlings.
Kabeeji ndị China (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) bụ nke genus Brassica ma bụrụ ihe oriri cruciferous a ma ama nke afọ nke a na-eto na obodo m (Wang et al., 2022; Yue et al., 2022). N'ime afọ ndị na-adịbeghị anya, ọnụ ọgụgụ mmepụta nke kọlịflawa ndị China anọgidewo na-amụba, na usoro ịkụ ihe agbanweela site na mkpụrụ osisi ọdịnala na-emepụta omenala na transplanting siri ike. Otú ọ dị, na usoro nke omenala seedling kpụ ọkụ n'ọnụ na transplanting, oke hypocotyl ibu na-achọsi ike na-amị leggy seedlings, na-akpata na-adịghị mma seedling mma. Ya mere, ịchịkwa oke hypocotyl ibu bụ ihe na-akpa ike na omenala seedling kpụ ọkụ n'ọnụ na transplanting nke Chinese kabeeji. Ugbu a, enwere ọmụmụ ole na ole na-ejikọta data transcriptomics na metabolomics iji chọpụta usoro nke elongation hypocotyl. Usoro ihe omimi nke chlorantazole na-ahazi mgbasawanye hypocotyl na kabeeji China amụbeghị. Anyị bu n'obi ịchọpụta mkpụrụ ndụ ihe nketa na ụzọ molekụla na-anabata uniconazole na-akpata hypocotyl dwarfing na kabeeji China. N'iji transcriptome na metabolomic analyses, yana yist otu-ngwakọ analysis, dual luciferase assay, na nje-induced gene silencing (VIGS) assay, anyị chọpụtara na uniconazole nwere ike ịkpata hypocotyl dwarfing na Chinese kabeeji site na igbochi lignin biosynthesis na Chinese kabeeji osisi. Nsonaazụ anyị na-enye nghọta ọhụrụ n'ime usoro nhazi nke molikula nke uniconazole na-egbochi elongation hypocotyl na kabeeji Chinese site na igbochi phenylpropanoid biosynthesis nke BrbZIP39-BrPAL4 modul. Nsonaazụ ndị a nwere ike inwe mmetụta bara uru dị mkpa maka imeziwanye ogo mkpụrụ osisi azụmahịa na inye aka n'ịhụ na mkpụrụ na ịdị mma nke akwụkwọ nri.
Agbanyere BrbZIP39 ORF zuru oke n'ime pGreenll 62-SK iji mepụta onye na-eme ihe, na mpempe akwụkwọ nkwalite BrPAL4 jikọtara ya na pGreenll 0800 luciferase (LUC) onye nta akụkọ iji mepụta mkpụrụ ndụ onye nta akụkọ. Onye na-ahụ maka mkpụrụ ndụ ihe na onye nta akụkọ ka agbanwere ya ka ọ bụrụ akwụkwọ ụtaba (Nicotiana benthamiana).
Iji dokwuo anya mmekọrịta nke metabolites na mkpụrụ ndụ ihe nketa, anyị mere metabolome nkwonkwo na nyocha transcriptome. Ntụle ịba ụba nke ụzọ KEGG gosipụtara na DEGs na DAM jikọtara ọnụ na ụzọ 33 KEGG (Nyocha 5A). N'ime ha, ụzọ "phenylpropanoid biosynthesis" bụ nke kachasị emejupụtara; ụzọ "photosynthetic carbon fixation", ụzọ "flavonoid biosynthesis", ụzọ "pentose-glucuronic acid interconversion", ụzọ "tryptophan metabolism" ụzọ, na "starch-sucrose metabolism" ụzọ bụkwa nke ukwuu ọgaranya. Maapụ nchịkọta okpomọkụ (Nyocha 5B) gosiri na DAM ndị metụtara DEGs kewara n'ọtụtụ dị iche iche, n'ime nke flavonoids bụ ụdị kachasị ukwuu, na-egosi na ụzọ "phenylpropanoid biosynthesis" na-arụ ọrụ dị mkpa na hypocotyl dwarfism.
Ndị na-ede akwụkwọ na-ekwupụta na e mere nchọpụta ahụ na enweghị mmekọrịta azụmahịa ma ọ bụ ego ọ bụla nwere ike ịkọwa dị ka esemokwu nwere ike ime.
Echiche niile ekwuputara n'akụkọ a bụ naanị nke onye odee, ọ bụghịkwa na-egosipụta echiche nke otu ndị mmekọ, ndị mbipụta, ndị editọ, ma ọ bụ ndị nyocha. Ngwaahịa ọ bụla enyochara n'ime akụkọ a ma ọ bụ nkwupụta nke ndị nrụpụta ha mere anaghị ekwe nkwa ma ọ bụ kwado ya site n'aka onye mbipụta ya.
Oge nzipu: Mar-24-2025