Kedu ihe dị iche n'etiti zeatin, Trans-zeatin na zeatin riboside? Kedu ihe bụ ngwa ha?

Isi ọrụ

1. Kwalite cell nkewa, tumadi nkewa nke cytoplasm;

2. Kwalite iche nwa osisi. Na omenala anụ ahụ, ọ na-emekọ ihe na auxin iji chịkwaa ọdịiche na nhazi nke mgbọrọgwụ na buds;

3. Kwalite mmepe nke mpụta buds, wepụ apical dominance, ma si otú a na-eduga n'ịmepụta a ọnụ ọgụgụ buru ibu nke adventitious buds na anụ ahụ omenala;

4. Igbu oge senescence nke akwukwo, belata nbibi nke chlorophyll na protein;

5. Gbajie mkpụrụ osisi dormancy, dochie ìhè izute ìhè chọrọ nke mkpụrụ dị ka ụtaba;

6. Mee ka parthenocarpy na ụfọdụ mkpụrụ osisi;

7. Kwalite guzobe nke mkpụrụ osisi mkpịrịsị: na mbepụ nsọtụ nke epupụta na ụfọdụ mosses, ọ nwere ike kwalite guzobe nke nwa osisi initials;

8. Na-akpali guzobe nduku tubers.

Ọ nwere naanị usoro trans ma nwee otu mmetụta dị kazeatin, ma na-arụ ọrụ siri ike.

Mmetụta ya dị nnọọ ka nke anti-zeatin. Ọ bụghị naanị na ọ nwere ọrụ ndị a kpọtụrụ aha n'elu nke zeatin, kamakwa ọ na-enwe mmetụta nke ịmegharị mkpụrụ ndụ ihe nketa na ọrụ metabolic.

Usoro ojiji

1. Kwalite germination nke callus (a ghaghị iji ya na auxin mee ihe), ntinye uche 1mg / L.

2. Kwalite ntọala mkpụrụ osisi, 1001 mg / L zeatin + 5001 mg / L GA3 + 201 mg / L NAA, fesaa na mkpụrụ osisi 10, 25, na 40 ụbọchị mgbe okooko gasịrị.

3. Maka akwụkwọ nri akwụkwọ ndụ akwụkwọ ndụ, fesaa na 201 mg / L iji gbuo oge ịcha edo edo.

Na mgbakwunye, ịgwọ ụfọdụ mkpụrụ osisi nwere ike ịkwalite germination; ọgwụgwọ n'oge seedling nwere ike ịkwalite uto.

1. Kwalite germination nke anụ ahụ callus (a ghaghị iji ya na auxin), na ntinye nke 1 ppm;

2. Kwalite ntọala mkpụrụ osisi, 100 ppm nke cytokinin + 500 ppm nke GA3 + 20 ppm nke NAA, fesa mkpụrụ osisi 10, 25, na 40 ụbọchị mgbe okooko gasịrị;

3. Igbu oge na-acha odo odo nke akwụkwọ nri, fesa 20 ppm;

1. Na omenala anụ ahụ nke osisi, ihe na-emekarị nke anti-cytokinin nucleoside bụ 1 mg / mL ma ọ bụ karịa.

2. N'ime usoro nhazi nke ihe ọkụkụ, ntinye nke anti-cytokinin nucleoside na-abụkarị 1 ppm ruo 100 ppm, na ntinye aka kpọmkwem dabere na ngwa ngwa na ụdị osisi. Dịka ọmụmaatụ, mgbe ị na-akwalite germination nke anụ ahụ callus, ntinye nke anti-cytokinin nucleoside bụ 1 ppm, ọ dịkwa mkpa ka ejiri ya na auxin.

3. Gbanyụọ mgbochi cytokinin nucleoside ntụ ntụ nke ọma na 2-5 mL nke 1 M NaOH (ma ọ bụ 1 M acetic acid ma ọ bụ 1 M KOH), wee gbakwunye mmiri na-ekpuchi okpukpu abụọ ma ọ bụ ultrapure mmiri iji kwadebe ihe nchekwa nchekwa nke 1 mg / mL ma ọ bụ elu elu. Kwuo mgbe ị na-agbakwunye mmiri iji hụ na agwakọta nke ọma. Ekwesịrị ịsacha ihe ngwọta nchekwa ahụ wee chekwaa oyi ka ọ ghara ịzere friza ugboro ugboro. Ghichaa ihe ngwọta nchekwa na omenala omenala na ntinye uche achọrọ, ma dozie ngwọta na-arụ ọrụ na ebe ahụ ma jiri ya ozugbo.

N'ikpeazụ, zeatin, abscisic acid na abscisic acid nucleotide nke ọ bụla nwere njirimara nke ya n'usoro nhazi, ọrụ na ngwa ọrụ. Agbanyeghị, ha niile na-arụ ọrụ dị ka ndị na-ahụ maka uto osisi ma na-ekere òkè dị ukwuu n'ịkwalite uto na mmepe osisi.