Site na mmepụta ihe karịrị tọn 700,000 kwa afọ, glyphosate bụ ọgwụ ogwu kachasị eji eme ihe ma bụrụkwa nke kachasị ukwuu n'ụwa. Mgbochi ahịhịa na ihe egwu nwere ike ibute gburugburu ebe obibi na ahụike mmadụ site na iji glyphosate eme ihe adọtala mmasị dị ukwuu.
Na Mee nke iri abụọ na itoolu, ndị otu Prọfesọ Guo Ruiting sitere na State Key Laboratory of Biocatalysis and Enzyme Engineering, nke Ụlọ Akwụkwọ nke Ndụ Sayensị nke Mahadum Hubei na ngalaba ógbè na nke minista hiwere, bipụtara akwụkwọ nyocha kachasị ọhụrụ na Journal of Hazardous Materials, na-enyocha nyocha mbụ nke ahịhịa barnyard. (A malignant paddy weed) nke aldo-keto reductase AKR4C16 na AKR4C17 na-akwalite usoro mmeghachi omume nke mmebi glyphosate, ma melite arụmọrụ mmebi nke glyphosate site na AKR4C17 site na mgbanwe molekul.
Ịba ụba nke iguzogide glyphosate.
Kemgbe e webatara ya n'afọ 1970, glyphosate abụrụla ihe a ma ama n'ụwa niile, ọ ghọkwara ọgwụ ogwu kacha dị ọnụ ala, nke a na-ejikarị eme ihe na nke kacha emepụta ihe. Ọ na-akpata nsogbu metabolic n'ime osisi, gụnyere ahịhịa, site na igbochi kpọmkwem 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), enzyme dị mkpa nke na-etinye aka na uto na metabolism nke osisi. na ọnwụ.
Ya mere, ịzụlite ihe ọkụkụ ndị na-eguzogide glyphosate na iji glyphosate n'ubi bụ ụzọ dị mkpa isi chịkwaa ahịhịa n'ọrụ ugbo nke oge a.
Agbanyeghị, site n'iji glyphosate eme ihe n'ụzọ na-ezighị ezi, ọtụtụ ahịhịa amụbaala nwayọọ nwayọọ ma nwee nnukwu ndidi glyphosate.
Tinyere nke ahụ, ihe ọkụkụ ndị na-eguzogide glyphosate nke gbanwere mkpụrụ ndụ ihe nketa ha enweghị ike ịgbari glyphosate, nke na-ebute nchịkọta na mbufe nke glyphosate n'ime ihe ọkụkụ, nke nwere ike gbasaa ngwa ngwa site na usoro nri ma tinye ahụike mmadụ n'ihe ize ndụ.
Ya mere, ọ dị mkpa ịchọpụta mkpụrụ ndụ ihe nketa nwere ike imebi glyphosate, ka e wee zụlite ihe ọkụkụ ndị na-eguzogide glyphosate dị elu nke nwere obere ihe fọdụrụ glyphosate.
Idozi usoro kristal na usoro mmeghachi omume katalytic nke enzymes ndị na-emebi glyphosate sitere na osisi
Na 2019, ndị otu nyocha nke China na Australia chọpụtara aldo-keto reductases abụọ nke glyphosate-degrading, AKR4C16 na AKR4C17, maka oge mbụ site na ahịhịa barnyard na-eguzogide glyphosate. Ha nwere ike iji NADP+ dị ka cofactor iji mebie glyphosate ka ọ bụrụ aminomethylphosphonic acid na glyoxylic acid na-adịghị egbu egbu.
AKR4C16 na AKR4C17 bụ enzymes mbụ akọpụtara na-emebi glyphosate nke sitere na evolushọn eke nke osisi mepụtara. Iji chọpụtakwuo usoro molekul nke mmebi ha nke glyphosate, ndị otu Guo Ruiting jiri X-ray crystallography nyochaa mmekọrịta dị n'etiti enzymes abụọ a na elu cofactor. Nhazi mgbagwoju anya nke mkpebi ahụ kpughere ụdị njikọ nke mgbagwoju anya ternary nke glyphosate, NADP+ na AKR4C17, ma tụọ aro usoro mmeghachi omume catalytic nke mmebi glyphosate nke AKR4C16 na AKR4C17 na-akwalite.
Nhazi nke AKR4C17/NADP+/glyphosate mgbagwoju anya na usoro mmeghachi omume nke mmebi glyphosate.
Mgbanwe nke molekul na-eme ka arụmọrụ mmebi nke glyphosate ka mma.
Mgbe ha nwetasịrị ụdị nhazi atọ dị mma nke AKR4C17/NADP+/glyphosate, ndị otu Prọfesọ Guo Ruiting nwetakwara protein mutant AKR4C17F291D nke nwere mmụba 70% na arụmọrụ mmebi nke glyphosate site na nyocha nhazi enzyme na imewe ezi uche.
Nyocha nke ọrụ glyphosate nke ndị na-agbanwe agbanwe AKR4C17.
“Ọrụ anyị na-ekpughe usoro molekul nke AKR4C16 na AKR4C17 na-akwalite mmebi nke glyphosate, nke na-atọ ntọala dị mkpa maka mgbanwe ọzọ nke AKR4C16 na AKR4C17 iji melite arụmọrụ mmebi ha nke glyphosate.” Onye dere akwụkwọ ahụ, Prọfesọ Dai Longhai nke Mahadum Hubei kwuru na ha wuru protein mutant AKR4C17F291D yana arụmọrụ mmebi glyphosate ka mma, nke na-enye ngwaọrụ dị mkpa maka ịzụlite ihe ọkụkụ transgenic dị elu na-eguzogide glyphosate yana obere ihe fọdụrụ glyphosate na iji nje bacteria injinia microbial iji mebie glyphosate na gburugburu ebe obibi.
A kọrọ na ndị otu Guo Ruiting anọwo na-eme nnyocha ogologo oge gbasara nyocha nhazi na mkparịta ụka usoro nke enzymes biodegradation, terpenoid synthases, na protein ndị na-emerụ ahụ na ihe ndị na-emerụ ahụ na gburugburu ebe obibi. Li Hao, onye nyocha ya bụ Yang Yu na onye nkuzi bụ Hu Yumei n'ime ndị otu ahụ bụ ndị mbụ dere akwụkwọ ahụ, Guo Ruiting na Dai Longhai bụkwa ndị edemede na-arụkọ ọrụ ọnụ.
Oge ozi: Jun-02-2022