Site na mmepụta ihe karịrị tọn 700,000 kwa afọ, glyphosate bụ ahịhịa ahịhịa kachasị eji na nke kachasị n'ụwa.Mgbochi ahihia na ihe egwu nwere ike ime na gburugburu ebe obibi na ahụike mmadụ kpatara site na iji glyphosate eme ihe na-adọta mmasị dị ukwuu.
Na Mee 29th, ndị otu Prọfesọ Guo Ruiting sitere na State Key Laboratory of Biocatalysis and Enzyme Engineering, nke ụlọ akwụkwọ nke Life Sciences nke Mahadum Hubei jikọtara ọnụ na ngalaba mpaghara na ndị ozi, bipụtara akwụkwọ nyocha kachasị ọhụrụ na Journal of Hazardous Materials, nyocha. mbụ nyocha nke barnyard ahịhịa.(Ahịhịa paddy ọjọọ) - nke sitere na aldo-keto reductase AKR4C16 na AKR4C17 na-eme ka usoro mmeghachi omume nke mmebi glyphosate na-eme ka ọ dịkwuo mma, ma na-emeziwanye arụmọrụ glyphosate nke AKR4C17 site na mgbanwe mkpụrụ ndụ.
Nguzogide glyphosate na-eto eto.
Kemgbe iwebata ya na 1970s, glyphosate na-ewu ewu n'ụwa nile, ma jiri nwayọọ nwayọọ ghọọ nke kachasị ọnụ ala, nke a na-ejikarị eme ihe na nke kachasị mma.Ọ na-ebute ọrịa metabolic na osisi, gụnyere ahịhịa, site na igbochi kpọmkwem 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), bụ isi enzyme na-etinye aka na uto osisi na metabolism.na ọnwụ.
Ya mere, ịzụlite ihe ọkụkụ transgenic na-eguzogide glyphosate na iji glyphosate n'ọhịa bụ ụzọ dị mkpa iji chịkwaa ahịhịa na ọrụ ugbo ọgbara ọhụrụ.
Otú ọ dị, site n'iji glyphosate na-eme ihe n'ọtụtụ ebe, ọtụtụ ahịhịa ejirila nwayọọ nwayọọ malite ma mepụta oke glyphosate.
Tụkwasị na nke ahụ, mkpụrụ ndụ ihe ọkụkụ na-eguzogide glyphosate enweghị ike imebi glyphosate, na-eme ka nchịkọta na ịnyefe glyphosate na ihe ọkụkụ, nke nwere ike gbasaa ngwa ngwa site na nri nri na-emerụ ahụ ike mmadụ.
Ya mere, ọ dị ngwa ngwa ịchọpụta mkpụrụ ndụ ihe nketa nwere ike imebi glyphosate, ka ọ wee na-akụ mkpụrụ osisi transgenic dị elu na-eguzogide glyphosate na obere glyphosate.
Na-edozi nhazi kristal na usoro mmeghachi omume catalytic nke enzymes na-emebi glyphosate sitere na osisi.
N'afọ 2019, ndị otu nyocha China na Australia chọpụtara aldo-keto reductases abụọ na-emebi glyphosate, AKR4C16 na AKR4C17, na nke mbụ site na ahịhịa ahịhịa na-eguzogide glyphosate.Ha nwere ike iji NADP + dị ka onye na-emekọ ihe iji weda glyphosate na aminomethylphosphonic acid na-adịghị egbu egbu na glyoxylic acid.
AKR4C16 na AKR4C17 bụ enzymes na-emebi emebi glyphosate nke izizi sitere na mmalite nke osisi.Iji nwetakwuo nyocha usoro mkpụrụ ndụ nke mmebi nke glyphosate ha, ndị otu Guo Ruiting jiri X-ray crystallography nyochaa mmekọrịta dị n'etiti enzymes abụọ a na cofactor dị elu.Usoro dị mgbagwoju anya nke mkpebi ahụ kpughere ụdị njikọ nke ternary complex nke glyphosate, NADP + na AKR4C17, wee tụpụta usoro mmeghachi omume catalytic nke AKR4C16 na AKR4C17-mediated glyphosate degeneration.
Nhazi nke AKR4C17/NADP+/glyphosate complex na mmeghachi omume nke mmebi glyphosate.
Mgbanwe nke molecular na-eme ka arụrụ arụ ọrụ nke glyphosate dịkwuo mma.
Mgbe ha nwetachara ụdị nhazi akụkụ atọ dị mma nke AKR4C17/NADP +/ glyphosate, ndị otu Prọfesọ Guo Ruiting nwetara protein mutant AKR4C17F291D na mmụba 70% na nbibi nke glyphosate site na nyocha nhazi enzyme na nhazi ezi uche.
Nyocha nke ọrụ na-eweda glyphosate nke ụmụ mutant AKR4C17.
"Ọrụ anyị na-ekpughe usoro molekụla nke AKR4C16 na AKR4C17 na-eme ka mmebi nke glyphosate, nke na-atọ ntọala dị mkpa maka ngbanwe ọzọ nke AKR4C16 na AKR4C17 iji melite arụmọrụ nke glyphosate ha."Onye na-ede akwụkwọ na-akwado akwụkwọ ahụ, Prọfesọ Dai Longhai nke Mahadum Hubei kwuru na ha rụrụ protein mutant AKR4C17F291D na arụmọrụ glyphosate dị mma, nke na-enye ngwá ọrụ dị mkpa maka ịmepụta ihe ọkụkụ transgenic dị elu nke na-eguzogide glyphosate na obere glyphosate fọdụrụnụ na iji microbial engineering. wedata glyphosate na gburugburu ebe obibi.
A na-akọ na ndị otu Guo Ruiting etinyela aka na nyocha nke nhazi usoro na mkparịta ụka usoro nke enzymes biodegradation, terpenoid synthases, na protein lekwasịrị anya ọgwụ na-egbu egbu na ihe na-emerụ ahụ na gburugburu ebe obibi.Li Hao, onye na-eme nchọpụta na-akpakọrịta Yang Yu na onye nkuzi Hu Yumei na otu ahụ bụ ndị na-ede akwụkwọ mbụ, na Guo Ruiting na Dai Longhai bụ ndị na-ede akwụkwọ na-emekọ ihe.
Oge nzipu: Jun-02-2022